Promega PubHub Articles

Full-size Humor

Tue, 31 Jan 2012 08:31:48 -0600

GoTaq® Long PCR Master Mix for Reliable Amplification of Long PCR Targets

Mon, 14 Nov 2011 11:25:26 -0600

This article describes the features and benefits of the GoTaq® Long PCR Master Mix. The Master Mix combines the proven performance of the GoTaq® Hot Start Polymerase with the higher fidelity of a proofreading polymerase.

Effective Determination of Cellular Viability in Microtissue Spheroids

Thu, 03 Nov 2011 13:19:19 -0500

This article describes ATP as a definitive biomarker of cell viability using the CellTiter-Glo Luminescent Cell Viability Assay and microtissue spheroids. Important cell-to-cell interactions can be recreated in vitro by 3-dimensional cell culture models.

BenchTop DNA Markers: A Convenient Option for Both Traditional Agarose and Precast Gel Systems

Wed, 26 Oct 2011 10:33:09 -0500

Here we analyzed the performance of five BenchTop DNA Markers in traditional agarose and precast gel systems. All five markers were compatible with both systems.

Comparison of luciferases as ATP sensors in the presence of inhibitors: CellTiter-Glo® Assay shows less compound interference than the Perkin Elmer ATPlite™ 1step assay

Fri, 30 Sep 2011 08:39:25 -0500

Here, we compare the abilities of the Promega CellTiter-Glo® Luminescent Cell Viability Assay and the Perkin Elmer ATPlite™ 1step assay to resist common luciferase inhibitors.

Screening for compounds that inhibit growth and sporulation of fungi and growth of algae using a luminescent assay

Thu, 29 Sep 2011 08:10:07 -0500

Authors describe application of the BacTiter-Glo™ Microbial Cell Viability Assay to assess cell viability in several species of algae, determine viability of fungal spores and screen for inhibitors of fungal sporulation.

tpub_063 Bioluminescent Assays for Detection of Lysine Deacetylase Activities

Tue, 27 Sep 2011 16:03:15 -0500

This article describes the HDAC-Glo™ I/II and SIRT-Glo™ Assays, which are useful for the detection of HDAC class I and II enzymes and the class III sirtuin enzymes, respectively. These simple, homogeneous “add-mix-measure” assays leverage bioluminescence to achieve robust and sensitive signal output useful from target validation to high-throughput screening. These assays can be multiplexed with viability and cytoxicity assays to correlate deacetylase inhibition with cellular fate.

Full-size Humor

Fri, 02 Sep 2011 14:09:14 -0500

Integrating Functional Proteomics Techniques to Rapidly Isolate and Characterize the Human Proteasome

Tue, 30 Aug 2011 09:02:49 -0500

How to use HaloTag® technology, and services from the Kazusa Institute and MS Bioworks to rapidly identify and characterize subunits in the human proteasome.

Screening and Profiling Kinase Inhibitors with a Luminescent ADP Detection Platform

Mon, 08 Aug 2011 13:11:56 -0500

This article describes the use of the luminescent ADP-Glo™ Assay to perform kinase inhibition selectivity profiles on four kinase inhibitors. Data include screening kinase inhibition activity of Stuarosporine for 70 Kinase Enzyme Systems.

Detecting Ozone-Induced Changes in Cellular Redox Balance via GSH/GSSG-Glo™ Assay

Fri, 01 Jul 2011 09:17:51 -0500

Steadily increasing levels of ambient ozone in urban areas correlate with increasing incidences of acute respiratory distress syndrome, asthma and lung cancer. In this article, researchers at Davidson College investigated the mechanisms by which lung cells respond to ozone-induced damage by measuring total and oxidized glutathione levels in rat pneumocytes exposed to ozone using the GSH/GSSG-Glo™ Assay.

Supplementary Information for Analyzing Protein Nucleic Acid Interactions Using HaloLink™ Protein Arrays

Fri, 24 Jun 2011 14:32:39 -0500

Supplementary Information containing detailed methods for the article describing HaloLink™ Protein Arrays and Protein:Nucleic Acid interactions.

Analyzing Protein:Nucleic Acid Interactions Using HaloLink™ Protein Arrays

Wed, 22 Jun 2011 15:04:48 -0500

This article uses the HaloLink™ Protein Array System to analyze protein:nucleic acid interactions. The Fusion proteins are generated using cell-free lysates and covalently attached to slide using the HaloTag® protein.

Protein Interactions Analysis Using Cell-Free Protein Expression Systems and HaloLink™ Protein Arrays

Mon, 20 Jun 2011 14:20:38 -0500

This article describes a rapid and flexible method to study protein-protein interactions using cell-free protein expression systems in combination with HaloLink™ Protein Arrays.

Purify RNA Transcribed In Vitro using the PureYield™ RNA Midiprep System

Thu, 16 Jun 2011 16:20:02 -0500

In this article, we use the PureYield™ RNA Midiprep System to purify RNA from in vitro transcription reactions. RNA transcribed using the RiboMAX™ Large Scale RNA Production System was purified successfully at milligram quantities without organic extractions or precipitations.