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Method of preparing a conditioned medium from a confluent stromal cell culture

Patent Number: 7678573
Publication Date: 2010-03-16
Inventors: Kadouri, Avinoam; Zipori, Dov; Merchav, Shoshana; Meretski, Shai

A method of preparing a stromal cell conditioned medium useful in expanding undifferentiated hemopoietic stem cells to increase the number of the hemopoietic stem cells is provided. The method comprising: (a) establishing a stromal cell culture in a stationary phase plug-flow bioreactor under continuous flow on a substrate in the form of a sheet, the substrate including a non-woven fibrous matrix forming a physiologically acceptable three-dimensional network of fibers, thereby expanding ...

Methods for preparing T-cells for cell therapy

Patent Number: 7678572
Publication Date: 2010-03-16
Inventor: Har-Noy, Michael

T-cells are generated with enhanced immunostimulatory capabilities for use in self therapy treatment protocols, by utilizing a biodegradable device with a biodegradable support that has one or more agents that are reactive to T-cell surface moieties. The biodegradable devices are mixed with the T-cells sufficiently so that the one or more agents cross-link with the T-cells' surface moieties and deliver a signal to the T-cells to enhance immunostimulatory ...

Procedure for the large-scale T-lymphocytes culture in a homogeneous system

Patent Number: 7678571
Publication Date: 2010-03-16
Inventors: De Bernardi, Nadia; Cavenaghi, Luigi; Trasciatti, Silvia; Nolli, Maria Luisa

The present invention refers to a procedure for the large-scale amplification of human lymphocytic cell lines for therapeutic use, consisting of a homogeneous culture system. The claimed invention also refers to the production of therapeutic doses of lymphocytic cells cultured ...

Human cell strains for protein production, provided by selecting strains with high intracellular protein and mutating with carcinogens

Patent Number: 7678570
Publication Date: 2010-03-16
Inventor: Kawahara, Hiroharu

A novel human cell strain enabling the continuous production of a desired protein with high efficiency, comprising a novel human cell strain established by transforming a human cell strain whose total intracellular protein weight is 0.1 to 1 mg per 1,000,000 cells; with the novel human cell strain being further characterized in that after a gene encoding a desired protein is transfected into it, the transfected cell is subsequently ...

Cloned genome of infectious hepatitis C virus strain HC-TN and uses thereof

Patent Number: 7678569
Publication Date: 2010-03-16
Inventors: Purcell, Robert H.; Bukh, Jens; Sakai, Akito; Emerson, Suzanne U.

Embodiments described herein include nucleic acid sequences, which encode hepatitis C virus of strain HC-TN, genotype 1a, proteins and polypeptides and fragments thereof. Use of these compositions, and diagnostics for HCV and in the development of screening assays for the identification of antiviral agents for HCV are also ...

Incubator

Patent Number: 7678568
Publication Date: 2010-03-16
Inventors: Etou, Daisuke; Tamaoki, Yuichi; Michida, Ayako; Busujima, Hiroki; Harada, Masaki; Houjou, Mikio; Yamada, Akihiko; Yamamoto, Hiroshi; Yokoi, Yasuhiko; Sakaguchi, Akira

The invention provides an incubator 1 wherein stackers 3 having a plurality of microplate accommodating portions are arranged in a chamber 11, and a microplate transport device 5 is arranged for transporting a microplate 31 within the chamber 11 and moving the microplate 31 into or out of a desired microplate accommodating portion. A camera 7 is provided on a position opposed to a microplate accommodating portion of an uppermost stage ...

Optical biosensor

Patent Number: 7678567
Publication Date: 2010-03-16
Inventors: Eto, Hideo; Oomiya, Kayoko; Uematsu, Ikuo; Tono, Ichiro

An optical biosensor has a total reflection plate so as to totally internally reflect and transmit an incident light, a first grating and a second grating disposed separately on the total reflection plate, and a sensing membrane that is containing an enzyme and a chromogenic reagent and is sandwiched by the first and second gratings on the total reflection ...

Device for chromatographic quantitative measurement

Patent Number: 7678566
Publication Date: 2010-03-16
Inventors: Miyoshi, Koji; Aga, Masahiro; Shigematsu, Kaoru

In a chromatography quantitative measuring apparatus according to the present invention, a beam applied from a light source to a chromatography test strip is formed into an elliptical shape by an optical means such as a cylindrical lens, a variation in absorbance that accompanies elution of a marker regent is detected while the elliptical beam is applied between a marker reagent hold part and a detection part, and a measurement is automatically started in a prescribed period of time since ...

Flow cell array and the utilization thereof for multianalyte determination

Patent Number: 7678565
Publication Date: 2010-03-16
Inventors: Abel, Andreas Peter; Bopp, Martin Andreas; Pawlak, Michael; Duveneck, Gert Ludwig; Schärer-Hernández, Nania Graciela; Schick, Eginhard; Ehrat, Markus; Schürmann-Mader, Eveline; Kresbach, Gerhard Matthias

A one- or two-dimensional arrangement of flow cells is provided, as part of an array of sample compartments, with at least one inlet and outlet for each sample compartment, formed by a base plate and a body, with an arrangement of spatial recesses corresponding to the (geometrical) arrangement of the sample compartments, combined with the base plate. The arrangement allows for supplying to or removing from the sample compartments, which can be arranged at a high quantity on a small base ...

Container with at least one electrode

Patent Number: 7678564
Publication Date: 2010-03-16
Inventors: Müller-Hartmann, Herbert; Siebenkotten, Gregor; Habig, Michael; Hoffmann, Peter

The invention relates to a container 20, 30 for receiving an aqueous solution, which is formed at least partially by an outer limit 21 forming an inner chamber 22, 32 for receiving the solution, and which comprises at least one area which acts as an electrode 25, 26, 33, 34 when an electric voltage is applied and a subsequent discharge occurs, wherein at least one electrode 25, 26, 33, 34 is made of a conductive synthetic material at least based on a ...

Method and apparatus for controlling air pressure in an organ or tissue container

Patent Number: 7678563
Publication Date: 2010-03-16
Inventors: Wright, David Walter; Brassil, John; Schein, Douglas

An organ perfusion apparatus and method monitor, sustain and/or restore viability of organs and preserve organs for storage and/or transport. Other apparatus include an organ transporter, an organ cassette and an organ diagnostic device. The apparatus and methods include the organ cassette with one or more openings configured to allow tubing to pass through the openings and be connected to the organ or tissue within the cassette, and including a pressure control device to allow pressure ...

Addressable nanopores and micropores including methods for making and using same

Patent Number: 7678562
Publication Date: 2010-03-16
Inventor: Ling, Xinsheng Sean

Featured are devices and systems embodying one or more electrically-addressable-solid-state nanopores useful for sensing and/or characterizing single macromolecules as well as sequencing DNA or RNA. In one aspect of the present invention, there is featured a linear or 2-D electrically-addressable array of nanopores, where the nanopores are located at points of intersections between V-shaped grooves formed in an upper surface of the insulating member and a V-shaped groove formed in a lower ...

Robust expression of a bioactive mammalian protein in chlamydomonas chloroplast

Patent Number: 7678561
Publication Date: 2010-03-16
Inventor: Mayfield, Stephen P.

Methods and compositions are disclosed to engineer chloroplast comprising heterologous mammalian genes via a direct replacement of chloroplast Photosystem II (PSII) reaction center protein coding regions to achieve expression of recombinant protein above 5% of total protein. When algae is used, algal expressed protein is produced predominantly as a soluble protein where the functional activity of the peptide is intact. As the host algae is edible, production of biologics in this organism ...

Δ 5 desaturase and its use in making polyunsaturated fatty acids

Patent Number: 7678560
Publication Date: 2010-03-16
Inventors: Xue, Zhixiong; Zhu, Quinn Qun; Damude, Howard Glenn; Pollak, Dana M. Walters

The present invention relates to a Δ5 desaturase, which has the ability to convert dihomo-γ-linolenic acid (DGLA; 20:3 ω-6) to arachidonic acid (ARA; 20:4 ω-6) and/or eicosatetraenoic acid (ETA; 20:4 ω-3) to eicosapentaenoic acid (EPA; 20:5 ω-3). Isolated nucleic acid fragments and recombinant constructs comprising such fragments encoding Δ5 desaturase along with a method of making long chain polyunsaturated fatty acids (PUFAs) using this Δ5 desaturase in oleaginous yeast are ...

Microbial composition and a process useful for the neutralization of alkaline waste-waters

Patent Number: 7678559
Publication Date: 2010-03-16
Inventors: Kumar, Anil; Kumar, Rita; Sharma, Alka; Gangal, Sharad; Makhijani, Santosh

The invention relates to a microbial composition for the neutralization of alkaline waste waters by biological means and a method of neutralization of alkaline waste waters using a synergistic mixture of the bacterial strains of Bacillus alkalophilus and Bacillus ...

Method for overexpression of zwitterionic polysaccharides

Patent Number: 7678558
Publication Date: 2010-03-16
Inventors: Weinacht, Katja G.; Comstock, Laurie E.; Coyne, Michael J.; Tzianabos, Arthur O.; Kasper, Dennis L.

The present invention is directed to methods for producing and selecting novel mutant strains of B. fragilis that constitutively express a particular capsular polysaccharide or only selected capsular polysaccharides; compositions directed to the novel mutant strains of B. fragilis that constitutively express a particular capsular polysaccharide or only selected capsular polysaccharides; improved methods for purification of individual capsular polysaccharides; and compositions ...

Methods for the accumulation and retention of immune-enhancing, bacterial-derived ribonucleotides (ORN) in bacteria

Patent Number: 7678557
Publication Date: 2010-03-16
Inventor: Marshall, William E.

The invention relates to methods for the accumulation and retention of immune-enhancing, bacterial-derived ribonucleotides smaller than 10,000 Daltons (ORN<10 kDa) in bacteria that are produced when bacteria are grown naturally, allowing the pH of the growth medium to become acidic. The immune-enhancing, bacterial-derived ribonucleotides (ORN) and bacteria comprising ORN are useful in the preparation of animal feed or animal drinking water as well as for use in enhancing the immune system ...

Beta-mannanase from coffee berry borer, , and uses thereof

Patent Number: 7678556
Publication Date: 2010-03-16
Inventors: Rose, Jocelyn; Acuña, Ricardo

The present invention relates to an isolated β-mannanase protein having an amino acid sequence which is 90% similar to the amino acid sequence of SEQ ID NO:1, as well as isolated polynucleotides encoding the β-mannanase protein, and isolated expression systems and host cells containing the polynucleotides. The present invention also relates to a method of recombinantly producing β-mannanase protein. Also disclosed is a method of degrading mannans and polysaccharides in plant material, ...

Production of crystalline short chain amylose

Patent Number: 7678555
Publication Date: 2010-03-16
Inventors: Norman, Barrie; Pedersen, Sven; Stanley, Keith D.; Richmond, Patricia A.

A process for producing a starch comprises treating a feed starch that comprises amylopectin with glucanotransferase to produce a chain-extended starch, and treating the chain-extended starch with a debranching enzyme to produce a starch product that comprises amylose fragments. At least about 38% by weight of the amylose fragments have a degree of polymerization (DP) of at least about ...

Nucleic acid shuffling

Patent Number: 7678554
Publication Date: 2010-03-16
Inventors: Bittker, Joshua A.; Liu, David R.

Disclosed is a method of altering a nucleic acid. The method includes fragmenting a parent nucleic acid strand to generate nucleic acid fragments. At least a subset of the fragments are ligated to generate shuffled nucleic acid strands. A selected strand is identified from the shuffled nucleic acid strands for a ...

Selection and use of lactic acid bacteria for reducing inflammation caused by

Patent Number: 7678553
Publication Date: 2010-03-16
Inventors: Connolly, Eamonn; Mollstam, Bo

Strains of Lactobacillus which have been selected for their capability of reducing gastrointestinal inflammation, such as that due to Helicobacter pylori, and products derived from these strains, including agents for treatment or prophylaxis of inflammation associated with Helicobacter pylori for administration to humans and include conditioned media in which the selected strains have grown and protein-containing extracts of the conditioned ...

Method for selecting therapeutic agents for cancer treatment

Patent Number: 7678552
Publication Date: 2010-03-16
Inventor: Kornblith, Paul L.

An improved system for screening a multiple of candidate therapeutic or chemotherapeutic agents for efficacy as to a specific patient, in which a tissue sample from the patient is harvested, cultured and separately exposed to a plurality of treatments and/or therapeutic agents for the purpose of objectively identifying. One particularly important tissue sample preparation technique is the initial preparation of cohesive multicellular particulates of the tissue sample. For assays concerning ...

Immunoassays for lamotrigine

Patent Number: 7678551
Publication Date: 2010-03-16
Inventors: Arabshahi, Lili; Ouyang, Anlong; Roberts, Mark; Wall, Melissa

Generally, the present invention relates to lamotrigine analogs that have substituents at the triazine 3-position and on the benzene 4-position and 5-position. The lamotrigine analogs can include immunogenic moieties that can be used to prepare anti-lamotrigine antibodies, or antigenic moieties that can be used in immunodiagnostic assays for lamotrigine. Also, the lamotrigine analog can include tracer moieties for detecting the presence or amount of the analog during an immunodiagnostic ...

FRET protease assays for botulinum serotype A/E toxins

Patent Number: 7678550
Publication Date: 2010-03-16
Inventors: Fernandez-Salas, Ester; Aoki, Kei Roger; Steward, Lance E.

The present invention provides clostridial toxin substrates useful in assaying for the protease activity of any clostridial toxin, including botulinum toxins of all serotypes as well as tetanus toxins. A clostridial toxin substrate of the invention contains a donor fluorophore; an acceptor having an absorbance spectrum overlapping the emission spectrum of the donor fluorophore; and a clostridial toxin recognition sequence that includes a cleavage site, where the cleavage site intervenes ...

Polyphenol inhibition of nucleoside diphosphate kinase-B activity and cancer metastasis

Patent Number: 7678549
Publication Date: 2010-03-16
Inventor: Buxton, Iain

Aggressive human tumor cells from disparate tissues were found to secrete nucleoside diphosphate kinase-B (NDPK-B) as a phosphoprotein into the extracellular environment. The secreted enzyme was capable of trasphosphorylation activity in the absence of a phosphoryl donor, thereby producing elevated level of extracellular ATP that plays a significant role in angiogenesis required for the growth of cancer cells and cancer metastasis. A series of structurally related non-nucleotide anticancer ...

High throughput assay systems and methods for identifying agents that alter expression of cellular proteins

Patent Number: 7678548
Publication Date: 2010-03-16
Inventors: Ficker, Eckhard; Brown, Arthur M.; Wible, Barbara A.

Disclosed are high throughput assay systems and methods for identifying agents that alter the level of expression of proteins in mammalian cells, particularly integral membrane ...

Velocity independent analyte characterization

Patent Number: 7678547
Publication Date: 2010-03-16
Inventors: Quake, Stephen; Eyal, Shulamit

The present invention provides apparatuses for determining velocity independent analyte characteristic parameters and methods for using the same. In one particular aspect, the present invention provides a velocity independent flow ...

Solid-state NMR method for screening cell membrane protein binding drug candidates

Patent Number: 7678546
Publication Date: 2010-03-16
Inventors: Brey, William W.; Smirnov, Alexej; Cross, Timothy; Chekmenev, Eduard Y

Disclosed is a method for detection of ligand-cell membrane protein binding by solid state NMR spectroscopy. The method starts by forming a lipid bilayer inside nanopores of an anodic aluminum oxide (AAO) substrate, the lipid bilayer containing a membrane protein sample. The AAO substrate is treated with multiple candidate ligands having potential binding affinity for the membrane protein. Solid-state NMR analysis is performed on the treated AAO/lipid preparation so as to generate an NMR ...

Polyelectrolyte multilayer films at liquid-liquid interfaces and methods for providing and using same

Patent Number: 7678545
Publication Date: 2010-03-16
Inventors: Lockwood, Nathan A.; Caruso, Frank; Tjipto, Elvira; Abbott, Nicholas; Cadwell, Katie D.

The present invention is directed to methods for providing a polyelectrolyte multilayer film at a liquid-liquid interface. Such methods include steps of sequentially-depositing layers of cationic and anionic polyelectrolytes at a liquid-liquid interface that is formed between immiscible first and second liquids whereby a polyelectrolyte multilayer film is provided at the liquid-liquid interface. In certain preferred embodiments, the first liquid is an aqueous solution and the second liquid ...

ADAM12, a novel marker for abnormal cell function

Patent Number: 7678544
Publication Date: 2010-03-16
Inventors: Wewer, Ulla M.; Christiansen, Michael; Nørgaard-Pedersen, Bent; Laigaard, Jennie; Fröhlich, Camilla; Spencer, Kevin

The present invention provides a method, an assay and a kit for providing an indication of abnormal cell function. It was surprisingly found that the change in the serum ADAM12 concentration in individuals was useful as a prognostic tool to predict the clinical outcome, complications and mortality following an abnormal cell function.

The present inventors describes ADAM12 as a overall general marker for abnormal cell function, and the present inventor for the first time demonstrate ...

Method for amplifying genomic DNA

Patent Number: 7678543
Publication Date: 2010-03-16
Inventors: Imai, Takashi; Iwakawa, Mayumi; Michikawa, Yuichi

A method for amplifying genomic DNA is provided. The method comprises the steps of: (1) incubating a cell-containing agarose solution at a pH of 9 to 12 and a temperature of 45 to 80° C. to produce a genomic DNA-dispersed agarose solution wherein 0.002 to 1 copies/5 microliter of single-stranded genomic DNA is dispersed; (2) solidifying the genomic DNA-dispersed agarose solution to produce a genomic DNA-dispersed agarose gel and neutralizing a pH of the gel; and (3) adding a DNA polymerase ...

Charge tags and the separation of nucleic acid molecules

Patent Number: 7678542
Publication Date: 2010-03-16
Inventors: Neri, Bruce P.; Takova, Tsetska Y.; Allawi, Hatim T.; Lyamichev, Victor; Skrzypczynski, Zbigniev; Wayland, Sarah R.; Prudent, James R.; Hall, Jeff G.

The present invention relates to novel phosphoramidites, including positive and neutrally charged compounds. The present invention also provides charge tags for attachment to materials including solid supports and nucleic acids, wherein the charge tags increase or decrease the net charge of the material. The present invention further provides methods for separating and characterizing molecules based on the charge differentials between modified and unmodified ...

Methods and compositions for the detection of a nucleic acid using a non-invasive cleavage reaction

Patent Number: 7678541
Publication Date: 2010-03-16
Inventors: Firmin, Andrew; Sorge, Joseph A.; Happe, Scott

The invention provides methods, compositions and kits for generating a signal indicative of the presence of a target nucleic acid sequence in a sample comprising forming a cleavage structure by incubating a sample comprising a target nucleic acid sequence with upstream and downstream oligonucleotides, and cleaving the cleavage structure with a nuclease to generate a signal. The presence of a detectable signal is indicative of the presence of a target nucleic acid sequence and a non-invasive ...

Template-directed assembly of receptor signaling complexes

Patent Number: 7678540
Publication Date: 2010-03-16
Inventors: Montefusco, David J.; Shrout, Anthony L.; Weis, Robert M.

Transmembrane receptors in the signaling pathways of bacterial chemotaxis systems influence cell motility by forming noncovalent complexes with the cytoplasmic signaling proteins to regulate their activity. The requirements for receptor-mediated activation of CheA, the principal kinase of the Escherichia coli chemotaxis signaling pathway, can be demonstrated using self-assembled clusters of a receptor fragment (CF) derived from the cytoplasmic domain of the aspartate receptor, Tar. ...

Arrays of biological membranes and methods and use thereof

Patent Number: 7678539
Publication Date: 2010-03-16
Inventors: Jonas, Steven J.; Lahiri, Joydeep; Fang, Ye; Frutos, Anthony G.; Kalal, Peter J.

The present invention overcomes the problems and disadvantages associated with prior art arrays by providing an array comprising a plurality of biological membrane microspots associated with a surface of a substrate that can be produced, used and stored, not in an aqueous environment, but in an environment exposed to air under ambient or controlled humidities. Preferably, the biological membrane microspots comprise a membrane bound protein. Most preferably, the membrane bound protein is a ...

Disinfection of biological fluids using asymmetric cyanine dyes

Patent Number: 7678538
Publication Date: 2010-03-16
Inventor: Wagner, Stephen J.

Asymmetric cyanine dyes of Formula I bind nucleic acid but not red blood cell membrane, and function as photosensitizers when rigidly bound but not when free in solution. Unbound dye thus causes minimal oxidative damage. The dyes do not substantially accumulate in red blood cells, thereby minimizing hemolysis due to oxidative damage. Biological fluids can be disinfected by mixing the fluid with these asymmetric cyanine dye that binds to nucleic acid, irradiating the mixture, and recovering ...

Plasmids and phages for homologous recombination and methods of use

Patent Number: 7674621
Publication Date: 2010-03-09
Inventors: Datta, Simanti; Court, Donald L.; Costantino, Nina

Lambda phages that can be used to introduce recombineering functions into host cells are disclosed. Also disclosed are plasmids that can be used to confer recombineering functions to a variety of strains of E. coli and to other bacteria, including Salmonella, Pseudomonas, Cyanobacteria, Spirochaetes. These plasmids and phages can be isolated in vitro and can be used to transform bacterial cells, such as gram negative ...

Derivation of terminally differentiated dopaminergic neurons from human embryonic stem cells

Patent Number: 7674620
Publication Date: 2010-03-09
Inventors: Ravindran, Geeta; Totey, Satish Mahadeorao

The present disclosure is directed to improved methods for efficiently producing neuroprogenitor cells and differentiated neural cells such as dopaminergic neurons and serotonergic neurons from pluripotent stem cells, for example human embryonic stem cells. Using the disclosed methods, cell populations containing a high proportion of cells positive for tyrosine hydroxylase, a specific marker for dopaminergic neurons, have been isolated. The neuroprogenitor cells and terminally ...

ADAM-9 modulators

Patent Number: 7674619
Publication Date: 2010-03-09
Inventors: Loo, Deryk T.; Mather, Jennie P.

The invention provides the identification and characterization of a disease and cancer-associated antigen, KID24. The invention also provides modulators of KID24, including a family of monoclonal antibodies that bind to antigen KID24, and methods of diagnosing and treating various human cancers and diseases associated with ...

Expression vector

Patent Number: 7674618
Publication Date: 2010-03-09
Inventor: Black, Amelia

This invention provides nucleic acids and expression vectors, and host cells transformed with the same, for providing high expression of a desired polypeptide. Also provided are methods of using the expression vectors, nucleic acids, and host cells which have been modified by these compositions, for increasing expression of a gene encoding a desired ...

MiRNA molecules isolated from human embryonic stem cell

Patent Number: 7674617
Publication Date: 2010-03-09
Inventors: Kim, Kye-Seong; Suh, Mi-Ra; Kim, Vit-Narry

The present invention relates to novel miRNA molecules, more particularly to novel miRNA molecules isolated from human embryonic stem cells. The miRNA molecules provided by the present invention can be usefully used as a molecular marker for early developmental stages of undifferentiated human embryonic stem cells. Also, the miRNA molecules of the present invention may play an important role in the regulation of mammalian embryonic stem cells. Therefore, the miRNA molecules can be usefully ...

Device and method for measuring properties of a sample

Patent Number: 7674616
Publication Date: 2010-03-09
Inventors: Navarro, Maria C.; Farnam, III, W. Edward

Test strips designed to prevent or reduce false results when measuring the condition of a sample are provided. The test strips can be used for analysis of samples by methods including electrical and optical measurements. The reagent test strips and methods are particularly suited for use in the detection of blood ...

Mechanical cartridge with test strip fluid control features for use in a fluid analyte meter

Patent Number: 7674615
Publication Date: 2010-03-09
Inventors: Ramel, Urs A.; Tay, Dillan

A structure for controlling fluid flow from a sample receiving pad into a lateral flow assay test strip, including: a lateral flow assay test strip; a sample pad abutting the lateral flow assay test strip; a pinch wall positioned to direct fluid flow from the sample pad to the lateral flow assay test strip; a top support structure positioned on top of the test strip; and a bottom support structure positioned underneath the test strip, wherein each of the top and bottom support structures ...

Method of optically resolving racemic alcohols with a bicyclooxaoctane or a bicycooxaoctene resolving reagents

Patent Number: 7674614
Publication Date: 2010-03-09
Inventors: Nemoto, Hisao; Shibuya, Masayuki

An optical resolving reagent comprising at least one of compounds represented by the following formulae (1) and (2) (1) (2) (wherein R¹to R⁸each represents hydrogen or C_1-20alkyl; R⁹represents optionally substituted C_1-20alkyl, optionally substituted C_1-20alkenyl, formyl, or acyl; and R¹⁰represents C_1-6alkyl; provided that the molecule represented by the formula (1) is of the cis configuration ...

Live bacterium preparation containing lactic acid bacterium as active ingredient and food containing lactic acid bacterium

Patent Number: 7674613
Publication Date: 2010-03-09
Inventor: Koga, Yasuhiro

Disclosed is a live bacterium preparation or food containing a lactic acid bacterium comprising a lactic acid bacterium, Lactobacillus salivarius, as an active ingredient. There is provided a live bacterium preparation and food containing a lactic acid bacterium that can prevent onset, recurrence and exacerbation of periodontal disease and/or dental caries caused by periodontopathic bacteria and cariogenic bacteria and can prevent generation of halitosis and maintain pH of saliva at ...

Infectious, chimeric hepatitis C virus, methods of producing the same and methods of use thereof

Patent Number: 7674612
Publication Date: 2010-03-09
Inventors: Jones, Christopher; Rice, Charles; Lindenbach, Brett D; Evans, Matthew J

The present invention provides infectious recombinant Hepatitis C Viruses (HCV), and vectors, cells and animals comprising the same. The present invention provides methods of producing infectious recombinant HCV, and their use in identifying anti-HCV therapeutic agents, as well as sequences of HCV associated with HCV ...

Modified ethylenediamine-N, N′-disuccinate: ethylenediamine lyase

Patent Number: 7674611
Publication Date: 2010-03-09
Inventors: Mizunashi, Wataru; Nakamura, Tetsuji; Akiyama, Takanori

The present invention provides a modified ethylenediamine-N,N′-disuccinate:ethylenediamine lyase. The present invention also provides a protein that comprises the amino acid sequence represented by SEQ ID NO: 1; or a protein that comprises an amino acid sequence derived from the amino acid sequence represented by SEQ ID NO: 1 by deletion, substitution, or addition of one or more amino acid residues, and has an ethylenediamine-N,N′-disuccinate:ethylenediamine lyase ...

Method and device for probing changes in a membrane by applying an in-plane electric field

Patent Number: 7674610
Publication Date: 2010-03-09
Inventor: Abeygunaratne, Thusara Sugat Chandra

The present invention is directed to disposing a membrane, for example, a biological cell membrane including amphiphilic lipids and proteins, or a liquid crystal membrane, between two electrical conductive walls having a height that is at least as great as the thickness of the membrane and, in particular, approximates the membrane thickness or height. The conductive walls are disposed on an electrically insulative base and can be approximately parallel to each other and perpendicular to the ...

Culture of and microorganisms derived therefrom

Patent Number: 7674609
Publication Date: 2010-03-09
Inventors: Anderson, Alistair James; Ratledge, Colin; Kanagachandran, Kanagasooriyam

Crypthecodinium cohnii, or microorganisms derived from Crypthecodinium cohnii, are grown in a culture medium including propionic acid. The propionic acid increases the production of one or more of dry cell weight, total lipid and docosahexaenoic ...

Saccharifying cellulose

Patent Number: 7674608
Publication Date: 2010-03-09
Inventors: Dadi, Anantharam Prasad; Schall, Constance Ann; Varanasi, Sasidhar

Dissolution, partial dissolution or softening of cellulose in an ionic liquid (IL) and its subsequent contact with anti-solvent produces regenerated cellulose more amorphous in structure than native cellulose, which can be separated from the IL/anti-solvent mixture by mechanical means such as simple filtration or centrifugation. This altered morphology of IL-treated cellulose allows a greater number of sites for enzyme adsorption with a subsequent enhancement of its saccharification. The ...