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Unnatural reactive amino acid genetic code additions

Patent Number: 8445446
Publication Date: 2013-05-21
Inventors: ; ; ;

This invention provides compositions and methods for producing translational components that expand the number of genetically encoded amino acids in eukaryotic cells. The components include orthogonal tRNAs, orthogonal aminoacyl-tRNA synthetases, pairs of tRNAs/synthetases and unnatural amino acids. Proteins and methods of producing proteins with unnatural amino acids in eukaryotic cells are also ...

Separation of pyrophosphate release and pyrophosphate detection

Patent Number: 8445412
Publication Date: 2013-05-21
Inventor:

The present technology relates to methods and systems for detection of pyrophosphate. As such, disclosed herein are methods and systems that permit improved pyrophosphate detection. Also disclosed herein are methods and systems which utilize improved pyrophosphate detection for nucleotide ...

Methods for producing biological substances in enzyme-deficient mutants of

Patent Number: 8445283
Publication Date: 2013-05-21
Inventors: ;

The present invention relates to methods of producing a heterologous biological substance, comprising: (a) cultivating a mutant of a parent Aspergillus niger strain in a medium suitable for the production of the heterologous biological substance, wherein (i) the mutant strain comprises a first nucleotide sequence encoding the heterologous biological substance and one or more second nucleotide sequences comprising a modification of glaA and at least one of the genes selected from the ...

Means to culture cochineal insects in an artificial medium

Patent Number: 8445282
Publication Date: 2013-05-21
Inventors: ;

A method is provided for culturing cochineal insects. In accordance with the method, a medium is created (101-109) from a mixture comprising a plant or cactus additive and a polymeric material. The medium is then inoculated (111) with a species selected from the group consisting of the genus Dactylopius. ...

In vitro exposure of immature oocytes to BDNF enhances generation of embryonic stem cells

Patent Number: 8445281
Publication Date: 2013-05-21
Inventors: ; ;

Compositions and methods are provided for enhancing the survival and promoting the maturation of mammalian oocytes, zygotes and preimplantation embryos. BDNF or BDNF agonists may be administered to an individual, or to cells in vitro, to enhance cellular maturation, embryo growth and fertilization. Accordingly, compositions comprising BDNF are herein presented for use in promoting in vivo oocyte maturation as well as for use as a component in culture media for promoting preimplantation ...

Method for creating perfusable microvessel systems

Patent Number: 8445280
Publication Date: 2013-05-21
Inventors: ; ;

A method for creating networks of perfusable microvessels in vitro. Cells including cell types capable of sprouting are seeded 1300 into a channel in a matrix at to activate competency 1304 of the cells for sprouting as microvessels based on the seeding density. The matrix channel is perfused with medium to allow parent vessels to form and for viability 1324. The parent vessels and matrix are incubated and perfused to provide for sprouting of microvessels from parent ...

Cultured cell construction containing spheroids of mesenchymal stem cells and utilization thereof

Patent Number: 8445279
Publication Date: 2013-05-21
Inventors: ; ; ; ; ; ;

Facing no ethical obstacle and easily to be isolated, multipotent concretely mesenchymal stem cells (MSCs) are one of the most powerful tools in reconstructive medicine. Here the inventors introduced 3D multicelluar spheroids culture construction based on photolithography and micropatterning techniques to improve multipotent differentiation efficiency of MSCs to adult cells. This invention, the 3D spheroid cultured construction for MSCs, leads to great improve of the differentiation ...

Process for producing decellularized biological tissues

Patent Number: 8445278
Publication Date: 2013-05-21
Inventors: ; ; ; ;

The present invention provides an electrophoretic system, apparatus, and method of use thereof for the preparation of a tissue-derived ...

Rejuvenation or preservation of germ cells

Patent Number: 8445277
Publication Date: 2013-05-21
Inventors: ;

Certain embodiments disclosed herein include, but are not limited to, at least one of compositions, methods, devices, systems, kits, or products regarding rejuvenation or preservation of germ cells or gametes. Certain embodiments disclosed herein include, but are not limited to, methods of modifying germ cells or gametes, or methods of administering modified germ cells or gametes to at least one biological ...

Device and method for growing and analyzing cells

Patent Number: 8445276
Publication Date: 2013-05-21
Inventors: ; ; ; ;

A device and method for analyzing cells includes a housing with a chamber, a barrier supported by a frame disposed within the chamber, and a plate arranged at a bottom surface of the housing interior of the chamber. The plate is adapted to receive and sustain cells and the barrier separates the plate into at least two contiguous separate areas. In some embodiments, a thin rubber strip is arranged at the bottom edge of the barrier, which facilitates control of the area in which each cell ...

Induction of dendritic cell development with macrophage-colony stimulating factor (M-CSF)

Patent Number: 8445275
Publication Date: 2013-05-21
Inventor:

A method of inducing dendritic cell (DC) development by administering Macrophage-Colony Stimulating Factor (M-CSF) is provided. M-CSF induces DCs to differentiate into Subtypes, for example plasmacytoid DCs and conventional DCs. Said differentiation is independent of Fms-like-Tyrosine-Kinase 3-Ligand (FL) and/or Granulocyte-Macrophage-Colony Stimulating Factor (GM-CSF). Induction with M-CSF can be achieved in vitro from hematopoietic precursors, such as bone marrow cells, or in vivo. In ...

Stem cell aggregate suspension compositions and methods of differentiation thereof

Patent Number: 8445273
Publication Date: 2013-05-21
Inventors: ; ; ;

The present invention relates to methods for production of undifferentiated or differentiated embryonic stem cell aggregate suspension cultures from undifferentiated or differentiated embryonic stem cell single cell suspensions and methods of differentiation ...

Method of stimulating NKT with 6″-amino-6″-deoxygalactosylceramides

Patent Number: 8445272
Publication Date: 2013-05-21
Inventors: ; ;

This invention relates to galactosylceramide ...

Processes and kits for determining multi-drug resistance of cells

Patent Number: 8445271
Publication Date: 2013-05-21
Inventors: ; ;

This invention relates to multi-drug resistance (MDR) in cells, and the use of certain xanthene compounds for determining drug resistance in cells and the effect of test compounds on cell membrane transport by the membrane transporters MDR1, MRP and BCRP. Processes and kits for making these determinations and measuring these effects are described and ...

Immortalized avian cell lines and use thereof

Patent Number: 8445270
Publication Date: 2013-05-21
Inventors: ; ;

The present invention relates to specific immortalized avian cell lines expressing telomerase reverse transcriptase (TERT), and exhibiting distinct biologics production patterns. More particularly, the present invention relates to immortalized avian cell line capable of either amplifying Flaviviridae but not capable of amplifying Vaccinia virus strain Copenhagen (VV-COP) nor Modified Vaccinia virus Ankara (MVA), or capable of amplifying both Flaviviridae and Poxviridae. The invention ...

Method for generating pluripotent stem cells

Patent Number: 8445269
Publication Date: 2013-05-21
Inventors: ;

The present invention relates to a method for generating pluripotent stem cells and to pluripotent stem cells generated from human ...

Her-2/neu DNA vaccine having anti-cancer activity

Patent Number: 8445268
Publication Date: 2013-05-21
Inventor:

The present invention relates to human Her-2/neu expressing plasmid constructs having anti-cancer activity and a DNA vaccine comprising same for preventing and/or treating cancer. The Her-2/neu DNA vaccines of the present invention can be effectively used as a therapeutic vaccine in reducing metastasis after tumor surgery or as a prophylactic vaccine for people with genetic high ...

Tyrosine-modified recombinant rAAV vector compositions and methods for use

Patent Number: 8445267
Publication Date: 2013-05-21
Inventor:

Disclosed are tyrosine-modified rAAV vectors, as well as infectious virions, compositions, and pharmaceutical formulations that comprise them. Also disclosed are methods of preparing and methods for using the disclosed tyrosine-phosphorylated capsid protein mutant rAAV vectors in a variety of diagnostic and therapeutic applications including in vivo and ex vivo gene therapy, and large-scale production of rAAV ...

Apparatus for judging cell detachment, method of judging cell detachment, and cell culture apparatus

Patent Number: 8445266
Publication Date: 2013-05-21
Inventors: ;

An apparatus for judging cell detachment that judges a state of detachment of cells that have been cultured within a cell culture container (cultured cells), includes: an image-capturing unit that captures an image of the cultured cells; and a detachment state judging unit that determines luminance information within the cell culture container based upon image capture data from the image-capturing unit, and judges that the culture cells are detached when the luminance information exceeds a ...

Reaction vessel and reaction controller

Patent Number: 8445265
Publication Date: 2013-05-21
Inventor:

The invention relates to a reaction vessel and a reaction controller, wherein temperature control of a liquid stored within the vessel can be performed with a high accuracy and faithful responsiveness. The reaction container and the reaction controller comprise one or a plurality of reaction chambers in which liquid is storable, and a wall that surrounds the reaction chamber, and the entirety or a part of the wall is formed by a temperature raising and lowering body that can raise or lower ...

Systems and methods for harvesting target particles of a suspension

Patent Number: 8445264
Publication Date: 2013-05-21
Inventors: ;

Tube and float systems and methods for isolating, enumerating, and harvesting target materials of a suspension are described. In one aspect, a tube and float system includes a filter embedded in a tube cap. The filter enables the passage of fluids but prevents the passage of the target materials. The tube and float system can be used to isolate and enumerate the target materials by centrifuging the tube and float system with the suspension to trap the target materials between the float and ...

Device for cellular electrophysiology sensor, cellular electrophysiology sensor using the device, and method for manufacturing the cellular electrophysiology sensor device

Patent Number: 8445263
Publication Date: 2013-05-21
Inventors: ;

A leakage current on a side surface of a sensor chip of a cell electrophysiological sensor is reduced. In order to do so, a sensor chip having a continuity hole and a chip holding part covering the side surface of the sensor chip are provided. The sensor chip includes silicon as a main component, and the chip holding part is made of glass. The chip holding part is adhesively bonded to the side surface of the sensor chip by glass welding. Thus, in the cell electrophysiological sensor device ...

Method for evaluating analyte

Patent Number: 8445262
Publication Date: 2013-05-21
Inventors: ; ;

In an analyte evaluation method for evaluating an analyte, AC voltage is applied between a substrate electrode on a substrate and a counter electrode, and signals obtained from a marker provided on an analyte bound to the substrate electrode are observed, wherein the frequency of the AC voltage is changed and the behavior of the average value of the marker signals is observed. A novel, highly-selective, low-noise method of evaluating a object of evaluation is thus ...

Apparatus for holding cells

Patent Number: 8445261
Publication Date: 2013-05-21
Inventors: ; ; ;

An apparatus for holding cells. The apparatus comprises a mechanism for incubating cells having a dynamically controlled environment in which the cells are grown, which are maintained in a desired condition and in which cells can be examined while the environment is dynamically controlled and maintained in the desired condition. The apparatus also comprises an imaging mechanism for automatically determining the state of an individual cell of the cells over time. The imaging mechanism is in ...

Apparatus and method for maintaining and/or restoring viability of organs

Patent Number: 8445260
Publication Date: 2013-05-21
Inventors: ; ; ; ; ; ; ;

An organ perfusion apparatus and method monitor, sustain and/or restore viability of organs and preserve organs for storage and/or transport. Other apparatus include an organ transporter, an organ cassette and an organ diagnostic device. In perfusion, organ perfusion pressure is preferably controlled in response to a sensor disposed in an end of tubing placed in the organ, by a pneumatically pressurized medical fluid reservoir, providing perfusion pressure fine tuning, overpressurization ...

Method and apparatus for treatmet of sludge

Patent Number: 8445259
Publication Date: 2013-05-21
Inventor:

An enhanced apparatus and a method for treating organic sludge is constructed such that the sludge is first dewatered with a first dewatering device; the dewatered sludge is passed through a thermal hydrolysis reactor to hydrolyze polymers contained in the dewatered sludge, the hydrolyzed sludge is passed through a digester to digest the hydrolyzed sludge anaerobically, the digested sludge is passed through a second dewatering device to dewater the anaerobically digested sludge to form ...

Recycling of waste material

Patent Number: 8445258
Publication Date: 2013-05-21
Inventor:

A process and apparatus for recycling municipal domestic waste comprises subjecting the waste to steam at 150° C.-200° C. at above atmospheric pressure but less than twice atmospheric pressure, After steam treatment, the resultant material is separated into constituent parts and biomass and/or plastics subjected to further treatment: The further treatment preferably produces bioethanol from the biomass and diesel from the plastics. As an alternative, some or all of the biomass may be ...

Production of branched-chain alcohols by photosynthetic microorganisms

Patent Number: 8445257
Publication Date: 2013-05-21
Inventor:

The present invention provides genes, polypeptides and expression constructs therefor, recombinant photosynthetic microorganisms, and method of use thereof, such as for the production of branched-chain alcohols (including 2-methyl-1-butanol, 3-methyl-1-butanol, and isobutanol) and derivatives thereof for a variety of ...

Liquid mycorrhiza compositions

Patent Number: 8445256
Publication Date: 2013-05-21
Inventors: ;

The present invention relates to liquid mycorrhiza compositions and to methods for colonizing a plant, grass, tree or shrub with one or more ...

Inoculants including bacteria for inducing production of volatile organic compounds in plants

Patent Number: 8445255
Publication Date: 2013-05-21
Inventors: ; ;

Disclosed are inoculants that include Bacillus bacteria and induce production of volatile organic compounds (VOCs) by a plant that has been treated with the ...

Regulated expression of antigen and/or regulated attentuation to enhance vaccine immunogenicity and/or safety

Patent Number: 8445254
Publication Date: 2013-05-21
Inventors: ; ; ;

The invention relates to compositions and methods for making and using recombinant bacteria that are capable of regulated attenuation and/or regulated expression of one or more antigens of ...

High performance nitrifying sludge for high ammonium concentration and low temperature wastewater treatment

Patent Number: 8445253
Publication Date: 2013-05-21
Inventor:

Compositions of bacteria which are effective for the nitrification of wastewater, particularly at low temperatures, are described. The compositions are comprised of 35 strains or populations of at least partially characterized isolated bacteria. These compositions may be used to treat wastewater contaminated with animal fecal waste and/or ammonia. In use, wastewater is contacted with the bacterial composition of the invention under aerobic conditions and for a period of time effective to ...

Method for producing functional compost, functional compost and compost for proliferation of filamentous fungus

Patent Number: 8445252
Publication Date: 2013-05-21
Inventor:

The method for producing functional compost according to the invention includes: inoculating a filamentous fungus with a function, such as the Coprinus curtus GM-21 strain (NITE BP-37) with a plant disease control function, into compost in an bacterial-activity-restricted state which is, for example, at least one state selected from the group consisting of a nutrient-restricted state, a pH-restricted state and a water content-restricted state; and cultivating the filamentous fungus ...

Rationally-designed single-chain meganucleases with non-palindromic recognition sequences

Patent Number: 8445251
Publication Date: 2013-05-21
Inventor:

Disclosed are rationally-designed, non-naturally-occurring meganucleases in which a pair of enzyme subunits having specificity for different recognition sequence half-sites are joined into a single polypeptide to form a functional heterodimer with a non-palindromic recognition sequence. The invention also relates to methods of producing such meganucleases, and methods of producing recombinant nucleic acids and organisms using such ...

Feed supplement

Patent Number: 8445250
Publication Date: 2013-05-21
Inventors: ; ; ; ; ;

The present invention relates to a feed supplement comprising a phytase and a lipolytic enzyme, wherein said lipolytic enzyme has lipase activity at a pH in the range of about pH1.5 to about ...

Sso7-polymerase conjugate proteins

Patent Number: 8445249
Publication Date: 2013-05-21
Inventor:

This invention provides Sso7-polymerase conjugates that exhibit improved activity in a polymerase ...

Hydrocarbon-forming oxidative decarbonylase enzyme, hydrocarbons produced thereby, and method of use

Patent Number: 8445248
Publication Date: 2013-05-21
Inventors: ;

The present disclosure relates to oxidative decarbonylase enzymes, methods of making hydrocarbons with such enzymes, hydrocarbons produced therefrom and uses thereof. More particularly, the present disclosure relates to isolated polypeptide sequences that are cytochrome P450 enzymes with oxidative decarbonylase activity and methods of their use to generate hydrocarbon products, such as ...

Stabilization of perhydrolases

Patent Number: 8445247
Publication Date: 2013-05-21
Inventors: ;

Disclosed herein are enzyme powders comprising a spray-dried formulation of at least one CE-7 esterase, at least one oligosaccharide excipient, and optionally at least one surfactant. Also disclosed herein is a process for producing peroxycarboxylic acids from carboxylic acid esters using the aforementioned enzyme powders. Further, disinfectant and laundry care formulations comprising the peracids produced by the processes described herein are ...

Flavin-binding glucose dehydrogenases

Patent Number: 8445246
Publication Date: 2013-05-21
Inventor:

A flavin-binding glucose dehydrogenase with a high substrate specificity for D-glucose. The flavin-binding glucose dehydrogenase which is derived from a microorganism belonging to the genus Mucor. The flavin-binding glucose dehydrogenase has a low reactivity for maltose, D-galactose and D-xylose compared to its reactivity for D-glucose, and therefore is relatively unaffected by these saccharide compounds. The flavin-binding glucose dehydrogenase is also relatively unaffected by ...

Mutant MT-SP1 proteases with altered substrate specificity or activity

Patent Number: 8445245
Publication Date: 2013-05-21
Inventor:

MT-SP1 mutein proteases with altered specificity for the target molecules they cleave can be used to treat human diseases, such as cancer. Cleaving VEGF or VEGFR at certain substrate sequences with wild-type and mutein MT-SP1 proteases can be used to treat pathologies associated with ...

Methods for increasing product yields

Patent Number: 8445244
Publication Date: 2013-05-21
Inventor:

A non-naturally occurring microbial organism includes a microbial organism having a reductive TCA or Wood-Ljungdahl pathway in which at least one exogenous nucleic acid encoding these pathway enzymes is expressed in a sufficient amount to enhance carbon flux through acetyl-CoA. A method for enhancing carbon flux through acetyl-CoA includes culturing theses non-naturally occurring microbial organisms under conditions and for a sufficient period of time to produce a product having acetyl-CoA ...

Hexose-pentose cofermenting yeast having excellent xylose fermentability and method for highly efficiently producing ethanol using the same

Patent Number: 8445243
Publication Date: 2013-05-21
Inventors: ;

Genetic recombinant yeast expressing xylose reductase (XR), (wild-type or mutant) xylitol dehydrogenase (XDH), and xylulokinase (XK) and a method for highly efficiently producing ethanol from xylose using the yeast are provided. Pichia stipitis-derived XR and (wild-type or modified-type) XDH genes and Saccharomyces cerevisiae-derived XK gene were introduced via chromosomal integration. Thus, a genetic recombinant yeast having a high xylose fermentation rate, being capable of ...

Perhydrolase providing improved specific activity

Patent Number: 8445242
Publication Date: 2013-05-21
Inventors: ;

An acetyl xylan esterase variant having perhydrolytic activity is provided for producing peroxycarboxylic acids from carboxylic acid esters and a source of peroxygen. More specifically, a Thermotoga maritima acetyl xylan esterase gene was modified using error-prone PCR and site-directed mutagenesis to create an enzyme catalyst characterized by an increase in specific activity. The variant acetyl xylan esterase may be used to produce peroxycarboxylic acids suitable for use in a ...

Process for the preparation of L-amino acids

Patent Number: 8445241
Publication Date: 2013-05-21
Inventors: ; ; ;

This application discloses methods for the preparation of L-amino acids, which comprises fermentation of a desired L-amino acid-producing bacteria in which at least the tal gene is amplified. In some embodiments, genes of the biosynthesis pathway of the desired L-amino acid are additionally ...

Termite enzymes and uses thereof for in vitro conversion of lignin-containing materials to fermentable products

Patent Number: 8445240
Publication Date: 2013-05-21
Inventors: ; ; ; ; ; ;

The disclosure provides isolated nucleic acid molecules derived from the gut of the termite R flavipes, recombinant nucleic acid molecules comprising a vector and an isolated heterologous nucleic acid molecule operably inserted therein, whereby, when transformed into an appropriate host cell system, the heterologous nucleic acid sequence is expressed as a polypeptide having an activity similar to that when expressed in the gut of the termite R. flavipes. The recombinant ...

Fermentation processes for cultivating streptococci and purification processes for obtaining CPS therefrom

Patent Number: 8445239
Publication Date: 2013-05-21
Inventors: ; ; ;

This invention is in the field of bacterial cultures and specifically relates to the optimization of culture conditions to improve the production of bacterial capsular polysaccharides from Streptococcus strains in fed batch culture and to novel purification methods suitable for production scale purification of bacterial capsular polysaccharides from Streptococcus strains resulting in higher levels of purity than previously obtained for production ...

Snap-back primers and detectable hairpin structures

Patent Number: 8445238
Publication Date: 2013-05-21
Inventors: ;

The present invention provides methods, compositions, and kits comprising snap-back primers used for forming 3′ hairpin structures, 5′ hairpin structures, and double hairpin structures. The hairpin structures may be used for detecting target sequences (e.g., such as small RNA target sequence), for detecting polymorphisms in target sequences (e.g., such as polymorphisms located near the 5′ or 3′ ends of the target sequence), or other nucleic acid characterization methods. In certain ...

RNA interference mediating small RNA molecules

Patent Number: 8445237
Publication Date: 2013-05-21
Inventors: ; ;

Double-stranded RNA (dsRNA) induces sequence-specific post-transcriptional gene silencing in many organisms by a process known as RNA interference (RNAi). Using a Drosophila in vitro system, we demonstrate that 19-23 nt short RNA fragments are the sequence-specific mediators of RNAi. The short interfering RNAs (siRNAs) are generated by an RNase III-like processing reaction from long dsRNA. Chemically synthesized siRNA duplexes with overhanging 3′ ends mediate efficient target RNA ...

Biomass pretreatment

Patent Number: 8445236
Publication Date: 2013-05-21
Inventors: ; ; ;

A method is provided for producing an improved pretreated biomass product for use in saccharification followed by fermentation to produce a target chemical that includes removal of saccharification and or fermentation inhibitors from the pretreated biomass product. Specifically, the pretreated biomass product derived from using the present method has fewer inhibitors of saccharification and/or fermentation without a loss in sugar ...

Process for the production of the toxins APXI or APXIII in a liquid culture medium under supply of air enriched in carbon dioxide

Patent Number: 8445235
Publication Date: 2013-05-21
Inventor:

The current invention pertains to a method to produce RTX-toxins ApxI or ApxIII by culturing Actinobacillus pleuropneumoniae bacteria in a liquid culturing medium that supports growth of the bacteria, characterized in that air is passed through the medium, wherein the air has a carbon dioxide content above normal atmospheric level to increase RTX-toxin production during the production phase of the ...