"Papers" via Brandon in Google Reader

The neural processing of voluntary completed, real and virtual violent and nonviolent computer game scenarios displaying predefined actions in gamers and nongamers.

2009-10-24T02:51:28Z

The neural processing of voluntary completed, real and virtual violent and nonviolent computer game scenarios displaying predefined actions in gamers and nongamers.

Soc Neurosci. 2009 Oct 12;:1-21

Authors: Regenbogen C, Herrmann M, Fehr T

Studies investigating the effects of violent computer and video game playing have resulted in heterogeneous outcomes. It has been assumed that there is a decreased ability to differentiate between virtuality and reality in people that play these games intensively. FMRI data of a group of young males with (gamers) and without (controls) a history of long-term violent computer game playing experience were obtained during the presentation of computer game and realistic video sequences. In gamers the processing of real violence in contrast to nonviolence produced activation clusters in right inferior frontal, left lingual and superior temporal brain regions. Virtual violence activated a network comprising bilateral inferior frontal, occipital, postcentral, right middle temporal, and left fusiform regions. Control participants showed extended left frontal, insula and superior frontal activations during the processing of real, and posterior activations during the processing of virtual violent scenarios. The data suggest that the ability to differentiate automatically between real and virtual violence has not been diminished by a long-term history of violent video game play, nor have gamers' neural responses to real violence in particular been subject to desensitization processes. However, analyses of individual data indicated that group-related analyses reflect only a small part of actual individual different neural network involvement, suggesting that the consideration of individual learning history is sufficient for the present discussion.

PMID: 19823959 [PubMed - as supplied by publisher]

Recent advances in brain-machine interfaces.

2009-10-24T02:50:41Z

Recent advances in brain-machine interfaces.

Neural Netw. 2009 Oct 17;

Authors: Isa T, Fetz EE, Müller KR

PMID: 19840893 [PubMed - as supplied by publisher]

The representation of tool use in humans and monkeys: common and uniquely human features.

2009-10-04T22:52:12Z

The representation of tool use in humans and monkeys: common and uniquely human features.

J Neurosci. 2009 Sep 16;29(37):11523-39

Authors: Peeters R, Simone L, Nelissen K, Fabbri-Destro M, Vanduffel W, Rizzolatti G, Orban GA

Though other species of primates also use tools, humans appear unique in their capacity to understand the causal relationship between tools and the result of their use. In a comparative fMRI study, we scanned a large cohort of human volunteers and untrained monkeys, as well as two monkeys trained to use tools, while they observed hand actions and actions performed using simple tools. In both species, the observation of an action, regardless of how performed, activated occipitotemporal, intraparietal, and ventral premotor cortex, bilaterally. In humans, the observation of actions done with simple tools yielded an additional, specific activation of a rostral sector of the left inferior parietal lobule (IPL). This latter site was considered human-specific, as it was not observed in monkey IPL for any of the tool videos presented, even after monkeys had become proficient in using a rake or pliers through extensive training. In conclusion, while the observation of a grasping hand activated similar regions in humans and monkeys, an additional specific sector of IPL devoted to tool use has evolved in Homo sapiens, although tool-specific neurons might reside in the monkey grasping regions. These results shed new light on the changes of the hominid brain during evolution.

PMID: 19759300 [PubMed - indexed for MEDLINE]

Decoding flexion of individual fingers using electrocorticographic signals in humans.

2009-10-04T22:51:47Z

Decoding flexion of individual fingers using electrocorticographic signals in humans.

J Neural Eng. 2009 Oct 1;6(6):66001

Authors: Kubánek J, Miller KJ, Ojemann JG, Wolpaw JR, Schalk G

Brain signals can provide the basis for a non-muscular communication and control system, a brain-computer interface (BCI), for people with motor disabilities. A common approach to creating BCI devices is to decode kinematic parameters of movements using signals recorded by intracortical microelectrodes. Recent studies have shown that kinematic parameters of hand movements can also be accurately decoded from signals recorded by electrodes placed on the surface of the brain (electrocorticography (ECoG)). In the present study, we extend these results by demonstrating that it is also possible to decode the time course of the flexion of individual fingers using ECoG signals in humans, and by showing that these flexion time courses are highly specific to the moving finger. These results provide additional support for the hypothesis that ECoG could be the basis for powerful clinically practical BCI systems, and also indicate that ECoG is useful for studying cortical dynamics related to motor function.

PMID: 19794237 [PubMed - as supplied by publisher]

Processing of Visual Signals for Direct Specification of Motor Targets and for Conceptual Representation of Action Targets in the Premotor Cortex.

2009-10-04T22:51:30Z

Processing of Visual Signals for Direct Specification of Motor Targets and for Conceptual Representation of Action Targets in the Premotor Cortex.

J Neurophysiol. 2009 Sep 30;

Authors: Yamagata T, Nakayama Y, Tanji J, Hoshi E

Previous reports have indicated that the premotor cortex (PM) uses visual information for either direct guidance of limb movements or indirect specification of action targets at a conceptual level. We explored how visual inputs signaling these two different categories of information are processed by PM neurons. Monkeys performed a delayed reaching task after receiving two different sets of visual instructions, one directly specifying the spatial location of a motor target (a direct spatial-target cue) and the other providing abstract information about the spatial location of a motor target by indicating whether to select the right or left target at a conceptual level (a symbolic action-selection cue). By comparing visual responses of PM neurons to the two sets of visual cues, we found that the conceptual action plan indicated by the symbolic action-selection cue was represented predominantly in dorsal PM (PMd) neurons with a longer latency (150 ms) whereas both PMd and ventral PM (PMv) neurons responded with a shorter latency (90 ms) when the motor target was directly specified with the direct spatial-target cue. We also found that excited, but not inhibited, responses of PM neurons to the direct spatial-target cue were biased toward contralateral preference. In contrast, responses to the symbolic action-selection cue were either excited or inhibited without laterality preference. Taken together, these results suggest that the PM constitutes a pair of distinct circuits for visually-guided motor act; one circuit, linked more strongly with PMd, carries information for retrieving action instruction associated with a symbolic cue, and the other circuit, linked with PMd and PMv, carries information for directly specifying a visuospatial position of a reach target.

PMID: 19793880 [PubMed - as supplied by publisher]

Control of a brain-computer interface without spike sorting.

2009-09-16T07:42:32Z

Control of a brain-computer interface without spike sorting.

J Neural Eng. 2009 Sep 1;6(5):55004

Authors: Fraser GW, Chase SM, Whitford A, Schwartz AB

Two rhesus monkeys were trained to move a cursor using neural activity recorded with silicon arrays of 96 microelectrodes implanted in the primary motor cortex. We have developed a method to extract movement information from the recorded single and multi-unit activity in the absence of spike sorting. By setting a single threshold across all channels and fitting the resultant events with a spline tuning function, a control signal was extracted from this population using a Bayesian particle-filter extraction algorithm. The animals achieved high-quality control comparable to the performance of decoding schemes based on sorted spikes. Our results suggest that even the simplest signal processing is sufficient for high-quality neuroprosthetic control.

PMID: 19721186 [PubMed - as supplied by publisher]

Bayesian inference of functional connectivity and network structure from spikes.

2009-09-16T07:42:22Z

Bayesian inference of functional connectivity and network structure from spikes.

IEEE Trans Neural Syst Rehabil Eng. 2009 Jun;17(3):203-13

Authors: Stevenson IH, Rebesco JM, Hatsopoulos NG, Haga Z, Miller LE, Körding KP

Current multielectrode techniques enable the simultaneous recording of spikes from hundreds of neurons. To study neural plasticity and network structure it is desirable to infer the underlying functional connectivity between the recorded neurons. Functional connectivity is defined by a large number of parameters, which characterize how each neuron influences the other neurons. A Bayesian approach that combines information from the recorded spikes (likelihood) with prior beliefs about functional connectivity (prior) can improve inference of these parameters and reduce overfitting. Recent studies have used likelihood functions based on the statistics of point-processes and a prior that captures the sparseness of neural connections. Here we include a prior that captures the empirical finding that interactions tend to vary smoothly in time. We show that this method can successfully infer connectivity patterns in simulated data and apply the algorithm to spike data recorded from primary motor (M1) and premotor (PMd) cortices of a monkey. Finally, we present a new approach to studying structure in inferred connections based on a Bayesian clustering algorithm. Groups of neurons in M1 and PMd show common patterns of input and output that may correspond to functional assemblies.

PMID: 19273038 [PubMed - indexed for MEDLINE]

Representation of goal and movements without overt motor behavior in the human motor cortex: a transcranial magnetic stimulation study.

2009-09-16T07:42:01Z

Representation of goal and movements without overt motor behavior in the human motor cortex: a transcranial magnetic stimulation study.

J Neurosci. 2009 Sep 9;29(36):11134-8

Authors: Cattaneo L, Caruana F, Jezzini A, Rizzolatti G

We recorded motor-evoked potentials (MEPs) to transcranial magnetic stimulation from the right opponens pollicis (OP) muscle while participants observed an experimenter operating two types of pliers: pliers opened by the extension of the fingers and closed by their flexion ("normal pliers") and pliers opened by the flexion of the fingers and closed by their extension ("reverse pliers"). In one experimental condition, the experimenter merely opened and closed the pliers; in the other, he grasped an object with them. In a further condition, the participants imagined themselves operating the normal and reverse pliers. During the observation of actions devoid of a goal, the MEP amplitudes, regardless of pliers used, reflected the muscular pattern involved in the execution of the observed action. In contrast, during the observation of goal-directed actions, the MEPs from OP were modulated by the action goal, increasing during goal achievement despite the opposite hand movements necessary to obtain it. During motor imagery, the MEPs recorded from OP reflected the muscular pattern required to perform the imagined action. We propose that covert activity in the human motor cortex may reflect different aspects of motor behavior. Imagining oneself performing tool actions or observing tool actions devoid of a goal activates the representation of the hand movements that correspond to the observed ones. In contrast, the observation of tool actions with a goal incorporates the distal part of the tool in the observer's body schema, resulting in a higher-order representation of the meaning of the motor act.

PMID: 19741119 [PubMed - indexed for MEDLINE]

A brain-machine interface instructed by direct intracortical microstimulation.

2009-09-16T07:41:31Z

A brain-machine interface instructed by direct intracortical microstimulation.

Front Integr Neurosci. 2009;3:20

Authors: O'Doherty JE, Lebedev MA, Hanson TL, Fitzsimmons NA, Nicolelis MA

Brain-machine interfaces (BMIs) establish direct communication between the brain and artificial actuators. As such, they hold considerable promise for restoring mobility and communication in patients suffering from severe body paralysis. To achieve this end, future BMIs must also provide a means for delivering sensory signals from the actuators back to the brain. Prosthetic sensation is needed so that neuroprostheses can be better perceived and controlled. Here we show that a direct intracortical input can be added to a BMI to instruct rhesus monkeys in choosing the direction of reaching movements generated by the BMI. Somatosensory instructions were provided to two monkeys operating the BMI using either: (a) vibrotactile stimulation of the monkey's hands or (b) multi-channel intracortical microstimulation (ICMS) delivered to the primary somatosensory cortex (S1) in one monkey and posterior parietal cortex (PP) in the other. Stimulus delivery was contingent on the position of the computer cursor: the monkey placed it in the center of the screen to receive machine-brain recursive input. After 2 weeks of training, the same level of proficiency in utilizing somatosensory information was achieved with ICMS of S1 as with the stimulus delivered to the hand skin. ICMS of PP was not effective. These results indicate that direct, bi-directional communication between the brain and neuroprosthetic devices can be achieved through the combination of chronic multi-electrode recording and microstimulation of S1. We propose that in the future, bidirectional BMIs incorporating ICMS may become an effective paradigm for sensorizing neuroprosthetic devices.

PMID: 19750199 [PubMed - in process]

Selective suppression of hippocampal ripples impairs spatial memory.

2009-09-16T07:41:21Z

Selective suppression of hippocampal ripples impairs spatial memory.

Nat Neurosci. 2009 Oct;12(10):1222-3

Authors: Girardeau G, Benchenane K, Wiener SI, Buzsáki G, Zugaro MB

Sharp wave-ripple (SPW-R) complexes in the hippocampus-entorhinal cortex are believed to be important for transferring labile memories from the hippocampus to the neocortex for long-term storage. We found that selective elimination of SPW-Rs during post-training consolidation periods resulted in performance impairment in rats trained on a hippocampus-dependent spatial memory task. Our results provide evidence for a prominent role of hippocampal SPW-Rs in memory consolidation.

PMID: 19749750 [PubMed - indexed for MEDLINE]

Active microelectronic neurosensor arrays for implantable brain communication interfaces.

2009-08-16T02:19:10Z

Active microelectronic neurosensor arrays for implantable brain communication interfaces.

IEEE Trans Neural Syst Rehabil Eng. 2009 Aug;17(4):339-45

Authors: Song YK, Borton DA, Park S, Patterson WR, Bull CW, Laiwalla F, Mislow J, Simeral JD, Donoghue JP, Nurmikko AV

We have built a wireless implantable microelectronic device for transmitting cortical signals transcutaneously. The device is aimed at interfacing a cortical microelectrode array to an external computer for neural control applications. Our implantable microsystem enables 16-channel broadband neural recording in a nonhuman primate brain by converting these signals to a digital stream of infrared light pulses for transmission through the skin. The implantable unit employs a flexible polymer substrate onto which we have integrated ultra-low power amplification with analog multiplexing, an analog-to-digital converter, a low power digital controller chip, and infrared telemetry. The scalable 16-channel microsystem can employ any of several modalities of power supply, including radio frequency by induction, or infrared light via photovoltaic conversion. As of the time of this report, the implant has been tested as a subchronic unit in nonhuman primates ( approximately 1 month), yielding robust spike and broadband neural data on all available channels.

PMID: 19502132 [PubMed - indexed for MEDLINE]

Control: conscious and otherwise.

2009-08-16T02:18:43Z

Control: conscious and otherwise.

Trends Cogn Sci. 2009 Aug;13(8):341-7

Authors: Suhler CL, Churchland PS

Social psychologists have shown human decisions to be sensitive to numerous ordinary, possibly nonconscious, situational contingencies, motivating the view that control is largely illusory, and that our choices are largely governed by such external contingencies. Against this view is evidence that self-control and goal-maintenance are regularly displayed by humans and other animals, and evidence concerning neurobiological processes that support such control. Evolutionarily speaking, animals with a robust capacity to exercise control - both conscious and nonconscious - probably enjoyed a selective advantage. Counterbalancing data thus point to an account of control that sees an important role for nonconscious control in action and goal maintenance. We propose a conceptual model of control that encompasses such nonconscious control and links in-control behavior to neurobiological parameters.

PMID: 19646918 [PubMed - indexed for MEDLINE]

The remapping of space in motor learning and human-machine interfaces.

2009-08-16T02:18:21Z

The remapping of space in motor learning and human-machine interfaces.

J Physiol Paris. 2009 Aug 6;

Authors: Mussa-Ivaldi FA, Danziger Z

Studies of motor adaptation to patterns of deterministic forces have revealed the ability of the motor control system to form and use predictive representations of the environment. One of the most fundamental elements of our environment is space itself. This article focuses on the notion of Euclidean space as it applies to common sensory motor experiences. Starting from the assumption that we interact with the world through a system of neural signals, we observe that these signals are not inherently endowed with metric properties of the ordinary Euclidean space. The ability of the nervous system to represent these properties depends on adaptive mechanisms that reconstruct the Euclidean metric from signals that are not Euclidean. Gaining access to these mechanisms will reveal the process by which the nervous system handles novel sophisticated coordinate transformation tasks, thus highlighting possible avenues to create functional human-machine interfaces that can make that task much easier. A set of experiments is presented that demonstrate the ability of the sensory-motor system to reorganize coordination in novel geometrical environments. In these environments multiple degrees of freedom of body motions are used to control the coordinates of a point in a two-dimensional Euclidean space. We discuss how practice leads to the acquisition of the metric properties of the controlled space. Methods of machine learning based on the reduction of reaching errors are tested as a means to facilitate learning by adaptively changing he map from body motions to controlled device. We discuss the relevance of the results to the development of adaptive human machine interfaces and optimal control.

PMID: 19665553 [PubMed - as supplied by publisher]

Neural encoding of auditory discrimination in ventral premotor cortex.

2009-08-16T02:18:06Z

Neural encoding of auditory discrimination in ventral premotor cortex.

Proc Natl Acad Sci U S A. 2009 Aug 25;106(34):14640-5

Authors: Lemus L, Hernández A, Romo R

Monkeys have the capacity to accurately discriminate the difference between two acoustic flutter stimuli. In this task, monkeys must compare information about the second stimulus to the memory trace of the first stimulus, and must postpone the decision report until a sensory cue triggers the beginning of the decision motor report. The neuronal processes associated with the different components of this task have been investigated in the primary auditory cortex (A1); but, A1 seems exclusively associated with the sensory and not with the working memory and decision components of this task. Here, we show that ventral premotor cortex (VPC) neurons reflect in their activities the current and remembered acoustic stimulus, their comparison, and the result of the animal's decision report. These results provide evidence that the neural dynamics of VPC is involved in the processing steps that link sensation and decision-making during auditory discrimination.

PMID: 19667191 [PubMed - indexed for MEDLINE]

Get aroused and be stronger: emotional facilitation of physical effort in the human brain.

2009-08-02T03:39:12Z

Get aroused and be stronger: emotional facilitation of physical effort in the human brain.

J Neurosci. 2009 Jul 29;29(30):9450-7

Authors: Schmidt L, Cléry-Melin ML, Lafargue G, Valabrègue R, Fossati P, Dubois B, Pessiglione M

Effort magnitude is commonly thought to reflect motivation, but little is known about the influence of emotional factors. Here, we manipulated the emotional state of subjects, via the presentation of pictures, before they exerted physical effort to win money. After highly arousing pictures, subjects produced more force and reported lower effort sensation, regardless of monetary incentives. Functional neuroimaging revealed that emotional arousal, as indexed by postscan ratings, specifically correlated with bilateral activity in the ventrolateral prefrontal cortex. We suggest that this region, by driving the motor cortex, constitutes a brain pathway that allows emotional arousal to facilitate physical effort.

PMID: 19641108 [PubMed - in process]

Implementation of spatial transformation rules for goal-directed reaching via gain modulation in monkey parietal and premotor cortex.

2009-08-02T03:38:51Z

Implementation of spatial transformation rules for goal-directed reaching via gain modulation in monkey parietal and premotor cortex.

J Neurosci. 2009 Jul 29;29(30):9490-9

Authors: Gail A, Klaes C, Westendorff S

Planning goal-directed movements requires the combination of visuospatial with abstract contextual information. Our sensory environment constrains possible movements to a certain extent. However, contextual information guides proper choice of action in a given situation and allows flexible mapping of sensory instruction cues onto different motor actions. We used anti-reach tasks to test the hypothesis that spatial motor-goal representations in cortical sensorimotor areas are gain modulated by the behavioral context to achieve flexible remapping of spatial cue information onto arbitrary motor goals. We found that gain modulation of neuronal reach goal representations is commonly induced by the behavioral context in individual neurons of both, the parietal reach region (PRR) and the dorsal premotor cortex (PMd). In addition, PRR showed stronger directional selectivity during the planning of a reach toward a directly cued goal (pro-reach) compared with an inferred target (anti-reach). PMd, however, showed stronger overall activity during reaches toward inferred targets compared with directly cued targets. Based on our experimental evidence, we suggest that gain modulation is the computational mechanism underlying the integration of spatial and contextual information for flexible, rule-driven stimulus-response mapping, and thereby forms an important basis of goal-directed behavior. Complementary contextual effects in PRR versus PMd are consistent with the idea that posterior parietal cortex preferentially represents sensory-driven, "automatic" motor goals, whereas frontal sensorimotor areas are stronger engaged in the representation of rule-based, "inferred" motor goals.

PMID: 19641112 [PubMed - in process]

Effects of a Body-oriented Response Measure on the Neural Substrate of Imagined Perspective Rotations.

2009-08-02T03:38:17Z

Effects of a Body-oriented Response Measure on the Neural Substrate of Imagined Perspective Rotations.

J Cogn Neurosci. 2009 Jul 30;

Authors: Wraga M, Flynn CM, Boyle HK, Evans GC

Abstract Previous behavioral studies suggest that response measures related to the body, such as pointing, serve to anchor participants to their physical body during mental rotation tasks in which their perspective must be shifted elsewhere (e.g., Wraga, M. Thinking outside the body: An advantage for spatial updating during imagined versus physical self-rotation. Journal of Experimental Psychology: Learning, Memory, and Cognition, 29, 993-1005, 2003). This study investigated whether such measures engage spatial and low-level cortical motor areas of the brain more readily than non-body-related measures. We directly compared activation found in two imagined perspective rotation tasks, using responses that varied in the degree to which they emphasized the human body. In the body minimize condition, participants imagined rotating themselves around an object and judged whether a prescribed part of the object would be visible from the imagined viewpoint. In the body maximize condition, participants imagined rotating around the object and then located the prescribed object part with respect to their bodies. A direct comparison of neural activation in both conditions revealed distinct yet overlapping neural regions. The body maximize condition yielded activation in low-level cortical motor areas such as premotor cortex and primary motor cortex, as well as bilateral spatial processing areas. The body minimize condition yielded activation in nonmotoric egocentric processing regions. However, both conditions showed activation in the parietal-occipital region that is thought to be involved in egocentric transformations. These findings are discussed in the context of recent hypotheses regarding the role of the body percept in imagined egocentric transformations.

PMID: 19642881 [PubMed - as supplied by publisher]

A real-time, 3-D musculoskeletal model for dynamic simulation of arm movements.

2009-08-02T03:35:35Z

A real-time, 3-D musculoskeletal model for dynamic simulation of arm movements.

IEEE Trans Biomed Eng. 2009 Apr;56(4):941-8

Authors: Chadwick EK, Blana D, van den Bogert AJ, Kirsch RF

Neuroprostheses can be used to restore movement of the upper limb in individuals with high-level spinal cord injury. Development and evaluation of command and control schemes for such devices typically require real-time, "patient-in-the-loop" experimentation. A real-time, 3-D, musculoskeletal model of the upper limb has been developed for use in a simulation environment to allow such testing to be carried out noninvasively. The model provides real-time feedback of human arm dynamics that can be displayed to the user in a virtual reality environment. The model has a 3-DOF glenohumeral joint as well as elbow flexion/extension and pronation/supination and contains 22 muscles of the shoulder and elbow divided into multiple elements. The model is able to run in real time on modest desktop hardware and demonstrates that a large-scale, 3-D model can be made to run in real time. This is a prerequisite for a real-time, whole-arm model that will form part of a dynamic arm simulator for use in the development, testing, and user training of neural prosthesis systems.

PMID: 19272926 [PubMed - indexed for MEDLINE]

Feature- and order-based timing representations in the frontal cortex.

2009-08-02T03:35:26Z

Feature- and order-based timing representations in the frontal cortex.

Neuron. 2009 Jul 30;63(2):254-66

Authors: Genovesio A, Tsujimoto S, Wise SP

We examined activity in the frontal cortex as monkeys performed a duration-discrimination task. Two stimuli, one red and the other blue, appeared sequentially on a video screen--in either order. Later, both stimuli reappeared, and to receive a reward the monkeys had to choose the stimulus that had lasted longer during its initial presentation. Some neurons encoded stimulus duration, but a larger number of cells represented their relative duration, which was encoded in three ways: whether the first or second stimulus had lasted longer; whether the red or blue stimulus had lasted longer; or, less commonly, as the difference between the two durations. As the monkeys' choice approached, the signal encoding which stimulus (red or blue) had lasted longer increased as the order-based signal dissipated. By representing stimulus durations and relative durations--both bound to stimulus features and event order--the frontal cortex could contribute to both temporal perception and episodic memory.

PMID: 19640483 [PubMed - indexed for MEDLINE]

Brain-computer interface research at the wadsworth center developments in noninvasive communication and control.

2009-07-18T19:39:18Z

Brain-computer interface research at the wadsworth center developments in noninvasive communication and control.

Int Rev Neurobiol. 2009;86:147-57

Authors: Krusienski DJ, Wolpaw JR

Brain-computer interface (BCI) research at the Wadsworth Center focuses on noninvasive, electroencephalography (EEG)-based BCI methods for helping severely disabled individuals communicate and interact with their environment. We have demonstrated that these individuals, as well as able-bodied individuals, can learn to use sensorimotor rhythms (SMRs) to move a cursor rapidly and accurately in one and two dimensions. We have also developed a practical P300-based BCI that enables users to access and control the full functionality of their personal computer. We are currently translating this laboratory-proved BCI technology into a system that can be used by severely disabled individuals in their homes with minimal ongoing technical oversight. Our comprehensive approach to BCI design has led to several innovations that are applicable in other BCI contexts, such as space missions.

PMID: 19607997 [PubMed - indexed for MEDLINE]