tag:blogger.com,1999:blog-1326907568089900322024-03-13T17:31:04.366+05:30BioSolutionsOnline repository of biological information which aims to create a knowledge base for students by the provision of animations and lectures.Julianhttp://www.blogger.com/profile/14658964544887212629noreply@blogger.comBlogger1345125tag:blogger.com,1999:blog-132690756808990032.post-82100426215656422092017-03-09T14:37:00.001+05:302021-09-15T20:16:07.034+05:30Meselson-Stahl Experiment (Semiconservative DNA replication)<div dir="ltr" style="text-align: left;" trbidi="on">
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The Meselson–Stahl experiment was an experiment by Matthew Meselson and Franklin Stahl with some additional help from a Canadian biologist, Mason MacDonald, and Canadian nuclear physicist, Amandeep Sehmbi, in 1958 which supported the hypothesis that DNA replication was semiconservative. In semiconservative replication, when the double stranded DNA helix is replicated, each of the two new double-stranded DNA helices consisted of one strand from the original helix and one newly synthesized. It has been called "the most beautiful experiment in biology."[1] Meselson and Stahl decided the best way to tag the parent DNA would be to change one of the atoms in the parent DNA molecule. Since nitrogen is found in the nitrogenous bases of each nucleotide, they decided to use an isotope of nitrogen to distinguish between parent and newly copied DNA. The isotope of nitrogen had an extra neutron in the nucleus, which made it heavier. <img alt="" height="0.5" src="https://i4.ytimg.com/vi/JcUQ_TZCG0w/default.jpg" width="1" /></div>
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Nitrogen is a major constituent of DNA. 14N is by far the most abundant isotope of nitrogen, but DNA with the heavier (but non-radioactive) 15N isotope is also functional.<br />
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E. coli was grown for several generations in a medium containing NH4Cl with 15N. When DNA is extracted from these cells and centrifuged on a salt density gradient, the DNA separates out at the point at which its density equals that of the salt solution. The DNA of the cells grown in 15N medium had a higher density than cells grown in normal 14N medium. After that, E. coli cells with only 15N in their DNA were transferred to a 14N medium and were allowed to divide; the progress of cell division was monitored by microscopic cell counts and by colony assay.</div>
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DNA was extracted periodically and was compared to pure 14N DNA and 15N DNA. After one replication, the DNA was found to have intermediate density. Since conservative replication would result in equal amounts of DNA of the higher and lower densities (but no DNA of an intermediate density), conservative replication was excluded. However, this result was consistent with both semiconservative and dispersive replication. Semiconservative replication would result in double-stranded DNA with one strand of 15N DNA, and one of 14N DNA, while dispersive replication would result in double-stranded DNA with both strands having mixtures of 15N and 14N DNA, either of which would have appeared as DNA of an intermediate density.</div>
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The authors continued to sample cells as replication continued. DNA from cells after two replications had been completed was found to consist of equal amounts of DNA with two different densities, one corresponding to the intermediate density of DNA of cells grown for only one division in 14N medium, the other corresponding to DNA from cells grown exclusively in 14N medium. This was inconsistent with dispersive replication, which would have resulted in a single density, lower than the intermediate density of the one-generation cells, but still higher than cells grown only in 14N DNA medium, as the original 15N DNA would have been split evenly among all DNA strands. The result was consistent with the semiconservative replication hypothesis.</div>
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<gwmw style="display: none;"></gwmw>Julianhttp://www.blogger.com/profile/14658964544887212629noreply@blogger.com0tag:blogger.com,1999:blog-132690756808990032.post-39213909932424064382017-03-09T14:29:00.001+05:302017-03-09T14:29:51.082+05:30Bacteriophage Animation<div align="justify">A bacteriophage (from 'bacteria' and Greek phagein, 'to eat') is any one of a number of viruses that infect bacteria. The term is commonly used in its shortened form, phage.</div><div align="justify">Typically, bacteriophages consist of an outer protein hull enclosing genetic material. The genetic material can be dsRNA, ssDNA, or dsDNA between 5 and 500 kilo base pairs long with either circular or linear arrangement. Bacteriophages are much smaller than the bacteria they destroy - usually between 20 and 200 nm in size.</div><img src="https://video.google.com/ThumbnailServer2?app=blogger&contentid=14cb4c684ce91aad&offsetms=5000&itag=w160&sigh=EbppJm9dirriwS0RlwrAEwInyrw" width="10" height="5" alt=" "><br />
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<div align="justify">Phages are estimated to be the most widely distributed and diverse entities in the biosphere. Phages are ubiquitous and can be found in all reservoirs populated by bacterial hosts, such as soil or the intestine of animals. One of the densest natural sources for phages and other viruses is sea water, where up to 109 virions per milliliter have been found at the surface, and up to 70% of marine bacteria may be infected by phages. They are also found in drinking water and in some foods, including fermented vegetables and meats e.g. pickles, salami, where they serve the function of controlling any growth of bacteria.</div><br />
<div align="justify">They have been used for over 60 years as an alternative to antibiotics in the former Soviet Union and Eastern Europe. They are now seen as a hope against multi drug resistant strains of many bacteria. However, in the case of MRSA, a phage infecting it produces the toxin and makes it more virulent and difficult to contain.</div><br />
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<b>Classification of phages</b><br />
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<div align="justify">The dsDNA tailed phages, or Caudovirales, account for 95% of all the phages reported in the scientific literature, and possibly make up the majority of phages on the planet. However, there are other phages that occur abundantly in the biosphere, phages with different virions, genomes and lifestyles. Phages are classified by the International Committee on Taxonomy of Viruses (ICTV) according to morphology and nucleic acid.</div><br />
<b>History</b><br />
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<div align="justify">Since ancient times, there have been documented reports of river water having the ability to cure infectious diseases, such as leprosy. In 1896, Ernest Hanbury Hankin reported that something in the waters of the Ganges and Jumna rivers in India had marked antibacterial action against cholera and could pass through a very fine porcelain filter. In 1915, British bacteriologist Frederick Twort, superintendent of the Brown Institution of London, discovered a small agent that infected and killed bacteria. He considered the agent either 1) a stage in the life cycle of the bacteria, 2) an enzyme produced by the bacteria themselves or 3) a virus that grew on and destroyed the bacteria. Twort's work was interrupted by the onset of World War I and shortage of funding. Independently, French-Canadian microbiologist Félix d'Hérelle, working at the Pasteur Institute in Paris, announced on September 3, 1917 that he had discovered "an invisible, antagonistic microbe of the dysentery bacillus". For d’Hérelle, there was no question as to the nature of his discovery: "In a flash I had understood: what caused my clear spots was in fact an invisible microbe ... a virus parasitic on bacteria." D'Hérelle called the virus a bacteriophage or bacteria-eater (from the Greek phagein meaning to eat). He also recorded a dramatic account of a man suffering from dysentery who was restored to good health by the bacteriophages. In 1926 in the Pulitzer-prizewinning novel Arrowsmith, Sinclair Lewis fictionalized the application of bacteriophages as a therapeutic agent. Also in the 1920s the Eliava Institute was opened in Tbilisi, Georgia to research this new science and put it into practice. In 2006 the UK Ministry of Defence took responsibility for a G8-funded Global Partnership Priority Eliava Project as a retrospective study to explore the potential of bacteriophages for the 21st century.</div><br />
<b>Replication</b><br />
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<div align="justify">Bacteriophages may have a lytic cycle or a lysogenic cycle, but a few viruses are capable of carrying out both. With lytic phages such as the T4 phage, bacterial cells are broken open (lysed) and destroyed after immediate replication of the virion. As soon as the cell is destroyed, the new bacteriophages viruses can find new hosts. Lytic phages are the kind suitable for phage therapy.</div><br />
<div align="justify">In contrast, the lysogenic cycle does not result in immediate lysing of the host cell. Those phages able to undergo lysogeny are known as temperate phages. Their viral genome will integrate with host DNA and replicate along with it fairly harmlessly, or may even become established as a plasmid. The virus remains dormant until host conditions deteriorate, perhaps due to depletion of nutrients, then the endogenous phages (known as prophages) become active. At this point they initiate the reproductive cycle resulting in lysis of the host cell. As the lysogenic cycle allows the host cell to continue to survive and reproduce, the virus is reproduced in all of the cell’s offspring.</div><br />
<div align="justify">Sometimes prophages may provide benefits to the host bacterium while they are dormant by adding new functions to the bacterial genome in a phenomenon called lysogenic conversion. A famous example is the conversion of a harmless strain of Vibrio cholerae by a phage into a highly virulent one, which causes cholera. This is why temperate phages are not suitable for phage therapy.</div><br />
<div class="separator" style="clear: both; text-align: center;"><iframe allowfullscreen='allowfullscreen' webkitallowfullscreen='webkitallowfullscreen' mozallowfullscreen='mozallowfullscreen' width='425' height='344' src='https://www.blogger.com/video.g?token=AD6v5dyKuPS0p_XWXxKs2sWPy4k0yRL2gglWA6Hp8bpZjpegpKozE7_xJaBqHDAv-Vg_M9yB1O13C93MRuNK1Pd9vA' class='b-hbp-video b-uploaded' frameborder='0'></iframe></div><b>Attachment and penetration</b><br />
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<div align="justify">To enter a host cell, bacteriophages attach to specific receptors on the surface of bacteria, including lipopolysaccharides, teichoic acids, proteins or even flagella. This specificity means that a bacteriophage can only infect certain bacteria bearing receptors that they can bind to, which in turn determines the phage's host range. As phage virions do not move independently, they must rely on random encounters with the right receptors when in solution (blood, lymphatic circulation, irrigation, soil water etc.).</div><br />
<div align="justify">Complex bacteriophages use a syringe-like motion to inject their genetic material into the cell. After making contact with the appropriate receptor, the tail fibers bring the base plate closer to the surface of the cell. Once attached completely, the tail contracts, possibly with the help of ATP present in the tail (Prescott, 1993), injecting genetic material through the bacterial membrane.</div><br />
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<b>Synthesis of proteins and nucleic acid</b><br />
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<div align="justify">Within minutes, bacterial ribosomes start translating viral mRNA into protein. For RNA-based phages, RNA replicase is synthesized early in the process. Proteins modify the bacterial RNA polymerase so that it preferentially transcribes viral mRNA. The host’s normal synthesis of proteins and nucleic acids is disrupted, and it is forced to manufacture viral products instead. These products go on to become part of new virions within the cell, helper proteins which help assemble the new virions, or proteins involved in cell lysis. Walter Fiers (University of Ghent, Belgium) was the first to establish the complete nucleotide sequence of a gene (1972) and of the viral genome of Bacteriophage MS2 (1976).</div><b><br />
Virion assembly</b><br />
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<div align="justify">In the case of the T4 phage, the construction of new virus particles involves the assistance of helper proteins. The base plates are assembled first, with the tails being built upon them afterwards. The head capsids, constructed separately, will spontaneously assemble with the tails. The DNA is packed efficiently within the heads. The whole process takes about 15 minutes.</div><br />
<b>Release of virions</b><br />
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<div align="justify">Phages may be released via cell lysis or by host cell secretion. In the case of the T4 phage, in just over twenty minutes after injection upwards of three hundred phages will be released via lysis within a certain timescale. This is achieved by an enzyme called endolysin which attacks and breaks down the peptidoglycan. In contrast, "lysogenic" phages do not kill the host but rather become long-term parasites and make the host cell continually secrete more new virus particles. The new virions bud off the plasma membrane, taking a portion of it with them to become enveloped viruses possessing a viral envelope. All released virions are capable of infecting a new bacterium.</div><br />
<b>Phage therapy</b><br />
<div align="justify">Phages were discovered to be anti-bacterial agents and put to use as such soon after they were discovered, with varying success. However, antibiotics were discovered some years later and marketed widely, popular because of their broad spectrum; also easier to manufacture in bulk, store and prescribe. Hence development of phage therapy was largely abandoned in the West, but continued throughout 1940s in the former Soviet Union for treating bacterial infections, with widespread use including the soldiers in the Red Army - much of the literature being in Russian or Georgian, and unavailable for many years in the West. This has continued after the war, with widespread use continuing in Georgia and elsewhere in Eastern Europe. There is much anecdotal evidence and case studies; There have also been clinical trials in Poland,</div><br />
<b>Bacteriophage in the environment</b><br />
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<div align="justify">Some time ago it was detected that phages are much more abundant in the water column of freshwater and marine habitats than previously thought and that they can cause significant mortality of bacterioplankton. Methods in phage community ecology have been developed to assess phage-induced mortality of bacterioplankton and its role for food web process and biogeochemical cycles, to genetically fingerprint phage communities or populations and estimate viral biodiversity by metagenomics. The release of lysis products by phages converts organic carbon from particulate (cells) to dissolved forms (lysis products), which makes organic carbon more bio-available and thus acts as a catalyst of geochemical nutrient cycles. Phages are not only the most abundant biological entities but probably also the most diverse ones. The majority of the sequence data obtained from phage communities has no equivalent in data bases. These data and other detailed analyses indicate that phage-specific genes and ecological traits are much more frequent than previously thought. In order to reveal the meaning of this genetic and ecological versatility, studies have to be performed with communities and at spatiotemporal scales relevant for microorganisms.</div><b><div align="justify"><br />
Bacteriophages and food fermentation</div></b><br />
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<div align="justify">A broad number of food products, commodity chemicals, and biotechnology products are manufactured industrially by large-scale bacterial fermentation of various organic substrates. Because enormous amounts of bacteria are being cultivated each day in large fermentation vats, the risk that bacteriophage contamination rapidly brings fermentations to a halt and cause economical setbacks is a serious threat in these industries. The relationship between bacteriophages and their bacterial hosts is very important in the context of the food fermentation industry. Sources of phage contamination, measures to control their propagation and dissemination, and biotechnological defense strategies developed to restrain phages are of interest. The dairy fermentation industry has openly acknowledged the problem of phage and has been working with academia and starter culture companies to develop defense strategies and systems to curtail the propagation and evolution of phages for decades.</div><b><br />
Other areas of use</b><br />
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<div align="justify">In August, 2006 the United States Food and Drug Administration (FDA) approved using bacteriophages on certain meats to kill the Listeria monocytogenes bacteria, giving them GRAS status (Generally Recognized As Safe). Government agencies in the West have for several years been looking to Georgia and the Former Soviet Union for help with exploiting phages for counteracting bioweapons and toxins e.g. Anthrax, Botulism. There are many developments with this amongst research groups in the US. Other uses include spray application in horticulture for protecting plants and vegetable produce from decay and the spread of bacterial disease. Other applications for bacteriophages are as a biocide for environmental surfaces e.g. hospitals - and as a preventative treatment for catheters and medical devices prior to use in clinical settings. The technology now exists for phages to be applied to dry surfaces e.g. uniforms, curtains - even sutures for surgery. Clinical trials reported in the Lancet show success in veterinary treatment of pet dogs with otitis. Phage display is a different use of phages. It is a powerful yet simple technique involving a library of phages. Each one has a slightly different peptide display. You can scan through the library to find a specific peptide that has a desired property - for example one that binds very tightly to Botulism toxin to neutralize it. Potentially it can then be used as a therapy.</div><br />
<div align="justify">Another use of bacteriophages is by the company Cambrios Technologies. Its founder, Dr. Angela Belcher, pioneered the use of the M13 bacteriophage to create nanowires and electrodes. One of her trials consisted of implanting gold and cobalt oxide in a bacteriophage to create a paper-thin electrode. The gold was for conductivity. The cobalt oxide was for the actual use of the battery</div>Julianhttp://www.blogger.com/profile/14658964544887212629noreply@blogger.com0tag:blogger.com,1999:blog-132690756808990032.post-91915854086480556462017-03-09T14:28:00.000+05:302017-03-09T14:28:45.078+05:30Lysogenic cycle Animation<div style="text-align: justify;">Lysogenic cycle, or lysogeny, is one of the two alternative life cycles of a virus inside a host cell, whereby the virus that has infected a cell attaches itself to the host DNA and, acting like an inert segment of the DNA, replicates when the host cell divides. This method of replication is contrasted with the lytic cycle, whereby the virus that has entered a cell takes over the cell's replication mechanism, makes viral DNA and viral proteins, and then lyses (breaks open) the cell, allowing the newly produced viruses to leave the now disintegrated host cell to infect other cells. While the lysogenic cycle causes no harm to the host cell, an induction event, such as exposure to ultraviolet light, can cause this latent stage to enter the lytic cycle.</div><div class="separator" style="clear: both; text-align: center;"><a href="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEi2EreV5Gw82sQt-nwX9mpAeDE6VIht5VqiVw1YXHnyhEGRoZ5DUUWN79N3X-3AWhvZOC4qkhy7hDtnUoi9bm9Fx1jtacMer6XcywR4R9-AiFuY2reJ8aBPpJQfzQvcoktNJgtWZc4dyJs/s1600/ThumbnailServer2.jpg" imageanchor="1" style="clear:left; float:left;margin-right:1em; margin-bottom:1em"><img border="0" height="1.20" width="1.60" src="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEi2EreV5Gw82sQt-nwX9mpAeDE6VIht5VqiVw1YXHnyhEGRoZ5DUUWN79N3X-3AWhvZOC4qkhy7hDtnUoi9bm9Fx1jtacMer6XcywR4R9-AiFuY2reJ8aBPpJQfzQvcoktNJgtWZc4dyJs/s200/ThumbnailServer2.jpg"></a></div><br />
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</script></div><div style="text-align: justify;">The lysogenic cycle is one strategy for replication of the virus without destruction of the host. The discovery of this cycle has important medical implications. Sometimes expression of the genes of a lysogenic bacteriophage can alter the phenotype of the host bacteria. This phenomenon, called lysogenic conversion, can have medical significance for humans. For example, the bacteria that causes diptheria, Corynebacterium diphtheriae, is harmless to humans unless it is infected by the phage β. Then the genes of the incorporated DNA of the bacteriophage induce the host bacteria to produce toxins.</div>Julianhttp://www.blogger.com/profile/14658964544887212629noreply@blogger.com0tag:blogger.com,1999:blog-132690756808990032.post-68487752738545871392016-12-18T15:47:00.000+05:302016-12-20T16:38:33.897+05:30Receptor-Mediated Endocytosis AnimationReceptor-mediated endocytosis (RME), also called clathrin-dependent endocytosis, is a process by which cells internalize molecules (endocytosis) by the inward budding of plasma membrane vesicles containing proteins with receptor sites specific to the molecules being internalized.<img alt=" " height="5" src="https://i4.ytimg.com/vi/PifagmJRLZ0/default.jpg" width="10"> <br />
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<b>Process<br />
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<div style="text-align: justify;">After the binding of a ligand to plasma membrane-spanning receptors, a signal is sent through the membrane, leading to membrane coating, and formation of a membrane invagination. The receptor and its ligand are then opsonized in clathrin-coated vesicles. Once opsonized, the clathrin-coated vesicle uncoats (a pre-requisite for the vesicle to fuse with other membranes) and individual vesicles fuse to form the early endosome. Since the receptor is internalized with the ligand, the system is saturable and uptake will decline until receptors are recycled to the surface. Common for biology.</div><br />
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<div class="v-wrap"><iframe allowfullscreen="" frameborder="0" height="315" src="https://www.youtube.com/embed/OejK9KAfFCY" width="420"></iframe></div><img border="0" height="0" src="https://i.ytimg.com/vi/OejK9KAfFCY/0.jpg" width="0" /><br />
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Function<br />
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<div style="text-align: justify;">The function of receptor-mediated endocytosis is diverse. It is widely used for the specific uptake of certain substances required by the cell (examples include LDL via the LDL receptor or iron via transferrin). The role of receptor-mediated endocytosis is also well recognized in the downregulation of transmembrane signal transduction. The activated receptor becomes internalised and is transported to late endosomes and lysosomes for degradation. However, receptor-mediated endocytosis is also actively implicated in transducing signals from the cell periphery to the nucleus. This became apparent when it was found that the association and formation of specific signaling complexes is required for the effective signaling of hormones (e.g. EGF). Additionally it has been proposed that the directed transport of active signaling complexes to the nucleus might be required to enable signaling as random diffusion is too slow and mechanisms permanently downregulating incoming signals are strong enough to shutdown signaling completely without additional signals transducing mechanisms.</div>Julianhttp://www.blogger.com/profile/14658964544887212629noreply@blogger.com0tag:blogger.com,1999:blog-132690756808990032.post-37637049475662589702016-12-11T14:53:00.000+05:302016-12-11T14:53:55.640+05:30What is Exocytotic Transport<div dir="ltr" style="text-align: left;" trbidi="on"><div style="text-align: justify;">Exocytosis is the cellular process in which intracellular vesicles in the cytoplasm fuse with the plasma membrane and release or "secrete" their contents into the extracellular space. Exocytosis can be constitutive (occurring all the time) or regulated. Constitutive exocytosis is important in transporting proteins like receptors that function in the plasma membrane. Regulated exocytosis is triggered when a cell receives a signal from the outside.<br />
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Many of the products that cells secrete function specifically for the tissue type in which the cells reside or are transmitted to more distant parts of the body. Most of these products are proteins that have gone through rigorous quality control and modification processes in the endoplasmic reticulum and Golgi membranes. It is in the trans -Golgi network, the "downstream" end of the Golgi apparatus, where cellular products are sorted and accumulate in exocytic vesicles.</div><br />
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<b>Mechanisms</b><br />
The mechanisms controlling regulated exocytosis were largely discovered in the 1990s. Contrary to early ideas, membranes normally do not fuse together spontaneously. This is due to the negative charges associated with the phospholipids that make up the lipid bilayer of the membranes of vesicles and organelles .<br />
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<div style="text-align: justify;">Membrane fusion requires energy and the interaction of special "adaptor" molecules present on both the vesicle and plasma membrane. The adapter molecules are highly selective and only allow vesicles to fuse with membranes of particular organelles, thus preventing harm to the cell. Once the appropriate adapter molecules bind to each other (docking), energy stored and released by ATP forms a fusion pore between the vesicle membranes and plasma membrane. The contents of the vesicle are released to the exterior of the cell (or the interior of an organelle) as the fusion pore widens. The vesicle ultimately becomes part of the plasma membrane or is recycled back to the cytoplasm.</div><br />
Purpose of Exocytosis<br />
Many cells in the body use exocytosis to release enzymes or other proteins that act in other areas of the body, or to release molecules that help cells communicate with one another. For instance, clusters of α-and β-cells in the islets of Langerhans in the pancreas secrete the hormones glucagon and insulin, respectively. These enzymes regulate glucose levels throughout the body. As the level of glucose rises in the blood, the β-cells are stimulated to produce and secrete more insulin by exocytosis. When insulin binds to liver or muscle, it stimulates uptake of glucose by those cells. Exocytosis from other cells in the pancreas also releases digestive enzymes into the gut.<br />
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Cells also communicate with each other more directly through the products that they secrete. For instance, a neuron cell relays an electrical pulse through the use of neurotransmitters . The neurotransmitters are stored in vesicles and lie next to the cytoplasmic face of the plasma membrane. When the appropriate signal is given, the vesicles holding the neurotransmitters must make contact with the plasma membrane and secrete their contents into the synaptic junction, the space between two neurons, for the other neuron to receive those neurotransmitters.<br />
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Components of the vesicle and extra neurotransmitter molecules are quickly taken up and recycled by the neuron to form new vesicles that are ready to send another pulse to an adjacent neuron. Neurons need to send many signals each second, which indicates how tight the controls are that regulate exocytosis.<br />
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The immune system also uses exocytosis to communicate information between cells. An immune cell can tell a virally infected cell that it must destroy itself to preserve other cells around it. A cell that is infected with a virus displays viral by-products on its surface, which is equivalent to the cell turning on red warning lights to attract immune cells.<br />
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Immune cells, such as the killer T cells that wander throughout the body, recognize the viral by-products and position themselves very close to the infected cell so that there is very little space between their plasma membranes. In a rapid succession, the killer T cells mobilize secretory vesicles filled with enzymes like perforin and granzyme B adjacent to the inner side of their plasma membranes. In response to a signal, the vesicles undergo exocytosis and release their contents. These enzymes then punch holes in the plasma membrane of the infected cell. This causes the cell to undergo self-destruction or apoptosis, also known as programmed cell death, to prevent further spread of the virus.</div>Julianhttp://www.blogger.com/profile/14658964544887212629noreply@blogger.com0tag:blogger.com,1999:blog-132690756808990032.post-88813162751018132422016-12-11T13:27:00.001+05:302016-12-11T13:27:18.202+05:30RNAi Transfection<iframe allowfullscreen='allowfullscreen' webkitallowfullscreen='webkitallowfullscreen' mozallowfullscreen='mozallowfullscreen' width='640' height='480' src='https://www.blogger.com/video.g?token=AD6v5dy5v5uAYkypvDP7P5c9-rxgJJnIAX7LB3_bNv9PwCQQoWfU92lCsHRWRtdg2O0QzjrKVw4Lv464lTzZETZbig' class='b-hbp-video b-uploaded' frameborder='0'></iframe><br />
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<div style="text-align: justify;">RNA interference (RNAi) technology is revolutionizing the biological discovery process as well as target discovery and validation. Using RNAi, you can turn gene expression "off", or knock it down, to better understand its function and role in disease. High-efficiency transfection is an essential first step for achieving effective gene knockdown.</div>Julianhttp://www.blogger.com/profile/14658964544887212629noreply@blogger.com0tag:blogger.com,1999:blog-132690756808990032.post-33204695978688399562016-12-08T15:53:00.000+05:302016-12-11T15:50:12.306+05:30What is Somatic cell nuclear transfer(SCNT)<div align="justify"><br />
Somatic cell nuclear transfer (SCNT) is a laboratory technique for creating an ovum with a donor nucleus . It can be used in embryonic stem cell research, or in regenerative medicine where it is sometimes referred to as "therapeutic cloning." It can also be used as the first step in the process of reproductive cloning.</div><br />
<div align="justify">In SCNT the nucleus, which contains the organism's DNA, of a somatic cell (a body cell other than a sperm or egg cell) is removed and the rest of the cell discarded. At the same time, the nucleus of an egg cell is removed. The nucleus of the somatic cell is then inserted into the enucleated egg cell. After being inserted into the egg, the somatic cell nucleus is reprogrammed by the host cell. The egg, now containing the nucleus of a somatic cell, is stimulated with a shock and will begin to divide. After many mitotic divisions in culture, this single cell forms a blastocyst (an early stage embryo with about 100 cells) with almost identical DNA to the original organism.</div><div class="v-wrap"><br />
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<strong>Process</strong><br />
<strong>SCNT in stem cell research</strong><br />
<div align="justify">Some researchers use SCNT in stem cell research. The aim of carrying out this procedure is to obtain stem cells that are genetically matched to the donor organism. Presently, no human stem cell lines have been derived from SCNT research.</div><br />
Human Embryonic Stem cell colony on mouse embryonic fibroblast feeder layer.<br />
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<div align="justify">A potential use of genetically-customized stem cells would be to create cell lines that have genes linked to the particular disease. For example, if a person with Parkinson's disease donated his or her somatic cells, then the stem cells resulting SCNT would have genes that contribute to Parkinson's disease. In this scenario, the disease-specific stem cell lines would be studied in order to better understand the disease.</div><br />
<div align="justify">In another scenario, genetically-customized stem cell lines would be generated for cell-based therapies to transplant to the patient. The resulting cells would be genetically identical to the somatic cell donor, thus avoiding any complications from immune system rejection.</div><br />
<div align="justify">Only a handful of the labs in the world are currently using SCNT techniques in human stem cell research. In the United States, scientists at the Harvard University Stem Cell Institute, the University of California San Francisco, and possibly Advanced Cell Technology are currently researching a technique to use somatic cell nuclear transfer to produce embryonic stem cells. In the United Kingdom, the Human Fertilisation and Embryology Authority has granted permission to research groups at the Roslin Institute and the Newcastle Centre for Life. SCNT may also be occurring in China.</div><br />
<div align="justify">In 2005, a South Korean research team led by Professor Hwang Woo-suk, published claims to have derived stem cell lines via SCNT, but supported those claims with fabricated data.Recent evidence has proved that he in fact created a stem cell line from a parthenote.</div><br />
<strong>SCNT in reproductive cloning</strong><br />
<div align="justify">This technique is currently the basis for cloning animals (such as the famous Dolly the sheep), and in theory could be used to clone humans. However, most researchers believe that in the foreseeable future it will not be possible to use this technique to produce a human clone that will develop to term.</div>Julianhttp://www.blogger.com/profile/14658964544887212629noreply@blogger.com0tag:blogger.com,1999:blog-132690756808990032.post-1026686262102412992016-12-02T16:45:00.001+05:302016-12-11T15:51:21.651+05:30How Embryonic Stem Cell Lines are Made<div dir="ltr" style="text-align: left;" trbidi="on"><div dir="ltr" style="text-align: left;" trbidi="on"><div style="text-align: justify;">Embryonic stem cells are derived from blastocysts — embryos that are about a week old. At this stage, the blastocyst has about 100 cells. Human blastocysts like this have been donated to research from in vitro fertilization clinics. In order to get embryonic stem cell lines, scientists remove cells from the inner cell mass region. These cells have the potential to develop into any type of cell in the body. Once the cells are removed, they are placed on a culture plate with nutrients and growth factors. The blastocyst is destroyed in this process. An embryonic cell line is established when these cells multiply and divide. Under the right conditions, these cell lines can be maintained indefinitely. By adding different growth factors, it is possible to induce these embryonic stem cells into developing into different cell types. These cells could someday be used in therapies to replace damaged cells and organs. </div><br />
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<div class="v-wrap"><iframe allowfullscreen="" frameborder="0" height="360" src="https://www.youtube.com/embed/3FyEpKfPkNg?rel=0" width="480"></iframe> <img alt=" " height="5" src="https://i4.ytimg.com/vi/3FyEpKfPkNg/default.jpg" width="10" /></div><br />
<a href="https://www.dnalc.org/content/c16/16991/embryonic-stem-cells.mp4">Download Video </a></div>Julianhttp://www.blogger.com/profile/14658964544887212629noreply@blogger.com0tag:blogger.com,1999:blog-132690756808990032.post-2110791040008761242016-12-01T15:36:00.000+05:302016-12-01T17:47:06.921+05:30Ligand gated ion channels structure and function<div dir="ltr" style="text-align: left;" trbidi="on"><div style="text-align: justify;">Ligand-gated ion channels (LGICs) are a group of transmembrane ion channel proteins which open to allow ions such as Na+, K+, Ca2+, or Cl- to pass through the membrane in response to the binding of a chemical messenger (i.e. a ligand),such as a neurotransmitter. <br />
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<iframe allowfullscreen="" frameborder="0" height="344" src="https://www.youtube.com/embed/Du-BwT0Ul2M?rel=0" title="YouTube video player" width="560"></iframe></div><div style="text-align: justify;"><br />
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<img alt=" " src="https://i4.ytimg.com/vi/Du-BwT0Ul2M/default.jpg" height="0.5" width="1"> These proteins are typically composed of at least two different domains: a transmembrane domain which includes the ion pore, and an extracellular domain which includes the ligand binding location (an allosteric binding site). This modularity has enabled a 'divide and conquer' approach to finding the structure of the proteins (crystallising each domain separately). The function of such receptors located at synapses is to convert the chemical signal of presynaptically released neurotransmitter directly and very quickly into a postsynaptic electrical signal. Many LGICs are additionally modulated by allosteric ligands, by channel blockers, ions, or the membrane potential. LGICs are classified into three superfamilies which lack evolutionary relationship: Cys-loop receptors, Ionotropic glutamate receptors and ATP-gated channels. LGICs can be contrasted with metabotropic receptors (which use second messengers), voltage-gated ion channels (which open and close depending on membrane potential), and stretch-activated ion channels (which open and close depending on mechanical deformation of the cell membrane)</div></div>Julianhttp://www.blogger.com/profile/14658964544887212629noreply@blogger.com0tag:blogger.com,1999:blog-132690756808990032.post-19769007592604365942016-09-12T19:59:00.000+05:302016-09-12T20:02:51.451+05:30Prozac: Selective Serotonin Reuptake Inhibitor<div style="text-align: justify;">This animation shows how Prozac® alleviates depression. It can also be used to illustrate in general how neuron cells communicate with each other and how a neurotransmitter sends a signal from one neuron to another.<br />
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<div style="text-align: justify;">Some people with depression have a shortage of serotonin, the "mood" neurotransmitter in the brain. The antidepressant Prozac®, a Selective Serotonin Reuptake Inhibitor (SSRI), can help correct this imbalance by increasing the brain's own supply of serotonin.<br />
</div><iframe width="560" height="344" src="https://www.youtube.com/embed/ElJaPZtSHoU?rel=0" frameborder="0" allowfullscreen></iframe><br />
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<div style="text-align: justify;">This animation shows how Prozac® acts as a selective inhibitor of Serotonin Reuptake Transporter Protein, thus alleviating depression. In the brain, serotonin is associated with transmission of thoughts and feelings. In a healthy person, an optimal concentration of serotonin is available at the synapse. The imbalance of this neurotransmitter triggers emotional symptoms, like depressed mood, or physical symptoms, like aches and pains.<br />
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<div style="text-align: justify;">The blue colored layers represent the trans-membrane structure of both pre- and post-synaptic areas (the upper and lower part of the screen, respectively). Red colored masses in the post-synaptic membrane represent serotonin receptors. There are other membrane proteins as well. Depression can occur when the serotonin transporter protein (a G-protein coupled receptor; shown in white in the pre-synaptic membrane) takes up a serotonin molecule before it has a chance to bind to the post-synaptic receptor. This process is known as reuptake. Prozac® blocks the reuptake of serotonin by disabling the transporter proteins. Consequently, more serotonin molecules will be available to the post-synaptic receptor and thus depression is relieved.</div>Julianhttp://www.blogger.com/profile/14658964544887212629noreply@blogger.comtag:blogger.com,1999:blog-132690756808990032.post-19762447316418048162016-09-10T00:31:00.000+05:302016-09-10T00:49:40.671+05:30Muscle contraction Animation<div align="justify">A muscle contraction (also known as a muscle twitch or simply twitch) occurs when a muscle fiber generates tension through the action of actin and myosin cross-bridge cycling. While under tension, the muscle may lengthen, shorten or remain the same. Though the term 'contraction' implies a shortening or reduction, when used as a scientific term referring to the muscular system contraction refers to the generation of tension by muscle fibers with the help of motor neurons. Locomotion in most higher animals is possible only through the repeated contraction of many muscles at the correct times. Contraction is controlled by the central nervous system (CNS), which comprises the brain and spinal cord. Voluntary muscle contractions are initiated in the brain, while the spinal cord initiates involuntary reflexes.</div><br />
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<b>Contractions, by muscle type</b> <br />
<div align="justify">For voluntary muscles, contraction occurs as a result of conscious effort originating in the brain. The brain sends signals, in the form of action potentials, through the nervous system to the motor neuron that innervates the muscle fiber. In the case of some reflexes, the signal to contract can originate in the spinal cord through a feedback loop with the grey matter. Involuntary muscles such as the heart or smooth muscles in the gut and vascular system contract as a result of non-conscious brain activity or stimuli endogenous to the muscle itself. Other actions such as locomotion, breathing, chewing have a reflex aspect to them; the contractions can be initiated consciously or unconsciously, but are continued through unconscious reflex. </div><br />
There are three general types of muscle tissues: <br />
<ul><li>Skeletal muscle (voluntary and involuntary) contractions </li>
<li>Cardiac muscle (involuntary) contractions </li>
<li>Smooth muscle (involuntary) contractions. </li>
<li> </li>
</ul><div align="justify">Skeletal and cardiac muscles are called striated muscle because of their striped appearance under a microscope which is due to the highly organized alternating pattern of A band and I band. </div><div align="justify">While nerve impulse profiles are, for the most part, always the same, skeletal muscles are able to produce varying levels of contractile force. This phenomenon can be best explained by Force Summation. Force Summation describes the addition of individual twitch contractions to increase the intensity of overall muscle contraction. This can be achieved in two ways: (1) by increasing the number and size of contractile units simultaneously, called multiple fiber summation, and (2) by increasing the frequency at which action potentials are sent to muscle fibers, called frequency summation. </div>Multiple Fiber Summation – When a weak signal is sent by the CNS to contract a muscle, the smaller motor units, being more excitable than the larger ones, are stimulated first. As the strength of the signal increases, more motor units are excited in addition to larger ones, with the largest motor units having as much as 50 times the contractile strength as the smaller ones. As more and larger motor units are activated, the force of muscle contraction becomes progressively stronger. A concept known as the size principle allows for a gradation of muscle force during weak contraction to occur in small steps, which then become progressively larger when greater amounts of force are required. <br />
<div align="justify">Frequency Summation - For skeletal muscles, the force exerted by the muscle is controlled by varying the frequency at which action potentials are sent to muscle fibers. Action potentials do not arrive at muscles synchronously, and during a contraction some fraction of the fibers in the muscle will be firing at any given time. Typically when a human is exerting a muscle as hard as they are consciously able, roughly one-third of the fibers in that muscle will be firing at once, but various physiological and psychological factors (including Golgi tendon organs and Renshaw cells) can affect that. This 'low' level of contraction is a protective mechanism to prevent avulsion of the tendon - the force generated by a 100% contraction of all fibers is sufficient to damage the body. </div><b>Skeletal muscle contractions</b> Skeletal muscles contract according to the sliding filament model: <br />
<ol><li>An action potential originating in the CNS reaches an alpha motor neuron, which then transmits an action potential down its own axon. </li>
<li>The action potential activates voltage-dependent calcium channels on the axon, and calcium rushes in. </li>
<li>Calcium causes vesicles containing the neurotransmitter acetylcholine to fuse with the plasma membrane, releasing acetylcholine into the synaptic cleft between the motor neuron terminal and the motor end plate of the skeletal muscle fiber. </li>
<li>The acetylcholine diffuses across the synapse and binds to and activates nicotinic acetylcholine receptor on the motor end plate. Activation of the nicotinic receptor opens its intrinsic sodium/potassium channel, causing sodium to rush in and potassium to trickle out. Because the channel is more permeable to sodium, the muscle fiber membrane becomes more positively charged, triggering an action potential. </li>
<li>The action potential spreads through the muscle fiber's network of T-tubules, depolarizing the inner portion of the muscle fiber. </li>
<li>The depolarization activates L-type voltage-dependent calcium channels (dihydropyridine receptors) in the T tubule membrane, which are in close proximity to calcium-release channels (ryanodine receptors) in the adjacent sarcoplasmic reticulum. </li>
<li>Activated voltage-gated calcium channels physically interact with calcium-release channels to activate them, causing the sarcoplasmic reticulum to release calcium. </li>
<li>The calcium binds to the troponin C present on the actin-containing thin filaments of the myofibrils. The troponin then allosterically modulates the tropomyosin. Normally the tropomyosin sterically obstructs binding sites for myosin on the thin filament; once calcium binds to the troponin C and causes an allosteric change in the troponin protein, troponin T allows tropomyosin to move, unblocking the binding sites. </li>
<li>Myosin (which has ADP and inorganic phosphate bound to its nucleotide binding pocket and is in a ready state) binds to the newly uncovered binding sites on the thin filament (binding to the thin filament is very tightly coupled to the release of inorganic phosphate). Myosin is now bound to actin in the strong binding state. The release of ADP and inorganic phosphate are tightly coupled to the power stroke (actin acts as a cofactor in the release of inorganic phosphate, expediting the release). This will pull the Z-bands towards each other, thus shortening the sarcomere and the I-band. </li>
<li>ATP binds myosin, allowing it to release actin and be in the weak binding state (a lack of ATP makes this step impossible, resulting in the rigor state characteristic of rigor mortis). The myosin then hydrolyzes the ATP and uses the energy to move into the "cocked back" conformation. In general, evidence (predicted and in vivo) indicates that each skeletal muscle myosin head moves 10-12 nm each power stroke, however there is also evidence (in vitro) of variations (smaller and larger) that appear specific to the myosin isoform. </li>
<li>Steps 9 and 10 repeat as long as ATP is available and calcium is present on thin filament. </li>
<li>While the above steps are occurring, calcium is actively pumped back into the sarcoplasmic reticulum. When calcium is no longer present on the thin filament, the tropomyosin changes conformation back to its previous state so as to block the binding sites again. The myosin ceases binding to the thin filament, and the contractions cease. </li>
</ol>The calcium ions leave the troponin molecule in order to maintain the calcium ion concentration in the sarcoplasm. The active pumping of calcium ions into the sarcoplasmic reticulum creates a deficiency in the fluid around the myofibrils. This causes the removal of calcium ions from the troponin. Thus the tropomyosin-troponin complex again covers the binding sites on the actin filaments and contraction ceases. <b>Contractions</b> <b>Concentric contraction</b> <br />
<div align="justify">A concentric contraction is a type of muscle contraction in which the muscles shorten while generating force. </div><div align="justify">During a concentric contraction, a muscle is stimulated to contract according to the sliding filament mechanism. This occurs throughout the length of the muscle, generating force at the musculo-tendinous junction, causing the muscle to shorten and changing the angle of the joint. In relation to the elbow, a concentric contraction of the biceps would cause the arm to bend at the elbow and hand to move from near to the leg, to close to the shoulder (a biceps curl). A concentric contraction of the triceps would change the angle of the joint in the opposite direction, straightening the arm and moving the hand towards the leg. </div><b>Eccentric contraction</b> <br />
<div align="justify">During an eccentric contraction, the muscle elongates while under tension due to an opposing force being greater than the force generated by the muscle. Rather than working to pull a joint in the direction of the muscle contraction, the muscle acts to decelerate the joint at the end of a movement or otherwise control the repositioning of a load. This can occur involuntarily (when attempting to move a weight too heavy for the muscle to lift) or voluntarily (when the muscle is 'smoothing out' a movement). Over the short-term, strength training involving both eccentric and concentric contractions appear to increase muscular strength more than training with concentric contractions alone. </div><div align="justify">During an eccentric contraction of the biceps muscle, the elbow starts the movement while bent and then straightens as the hand moves away from the shoulder. During an eccentric contraction of the triceps muscle, the elbow starts the movement straight and then bends as the hand moves towards the shoulder. Desmin, titin, and other z-line proteins are involved in eccentric contractions, but their mechanism is poorly understood in comparison to cross-bridge cycling in concentric contractions. </div><div align="justify">Muscles undergoing heavy eccentric loading suffer greater damage when overloaded (such as during muscle building or strength training exercise) as compared to concentric loading. When eccentric contractions are used in weight training they are normally called "negatives". During a concentric contraction muscle fibers slide across each other pulling the Z-lines together. During an eccentric contraction, the filaments slide past each other the opposite way, though the actual movement of the myosin heads during an eccentric contraction is not known. Exercise featuring a heavy eccentric load can actually support a greater weight (muscles are approximately 10% stronger during eccentric contractions than during concentric contractions) and also results in greater muscular damage and delayed onset muscle soreness one to two days after training. Exercise that incorporates both eccentric and concentric muscular contractions (i.e. involving a strong contraction and a controlled lowering of the weight) can produce greater gains in strength than concentric contractions alone. The caveat for this is that heavy eccentric contractions can easily lead to over-training since they are so demanding. </div><b> Eccentric contractions in movement</b> <br />
<div align="justify">Eccentric contractions normally occur as a braking force in opposition to a concentric contraction to protect joints from damage. During virtually any routine movement, eccentric contractions assist in keeping motions smooth, but can also slow rapid movements such as a punch or throw. Part of training for rapid movements such as pitching during baseball involves reducing eccentric braking allowing a greater power to be developed throughout the movement. </div><div align="justify">Eccentric contractions are being researched for their ability to speed rehab of weak or injured tendons. Achilles tendinitis has been shown to benefit from high load eccentric contractions. </div><b>Isometric contraction</b> <br />
<div align="justify">An isometric contraction of a muscle generates force without changing length. An example can be found in the muscles of the hand and forearm grip an object; the joints of the hand do not move but muscles generate sufficient force to prevent the object from being dropped. </div>Julianhttp://www.blogger.com/profile/14658964544887212629noreply@blogger.com0tag:blogger.com,1999:blog-132690756808990032.post-74079691678790009222016-09-07T16:20:00.000+05:302016-09-09T22:53:40.629+05:30Proliferation of T cell by IL-10<div dir="ltr" style="text-align: left;" trbidi="on">
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Human IL-10 has been reported previously to inhibit the secretion of IFN-gamma in PBMC. In this study, we have found that human IL-10 inhibits T cell proliferation to either mitogen or anti-CD3 mAb in the presence of accessory cells. Inhibited T cell growth by IL-10 was associated with reduced production of IFN-gamma and IL-2. Studies of T cell subset inhibition by human IL-10 showed that CD4+, CD8+, CD45RA high, and CD45RA low cells are all growth inhibited to a similar degree. Dose response experiments demonstrated that IL-10 inhibits secretion of IFN-gamma more readily than T cell proliferation to mitogen. In addition, IL-2 and IL-4 added exogenously to IL-10 suppressed T cell cultures reversed completely the inhibition of T cell proliferation, but had little or no effect on inhibition of IFN-gamma production. Thus, in addition to its previously reported biologic properties, IL-10 inhibits human T cell proliferation and IL-2 production in response to mitogen. Inhibition of IFN-gamma production by IL-10 appears to be independent of the cytokine effect of IL-2 production.</div>
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Julianhttp://www.blogger.com/profile/14658964544887212629noreply@blogger.com0tag:blogger.com,1999:blog-132690756808990032.post-58592329179409096432015-10-25T22:10:00.000+05:302016-09-09T22:46:58.365+05:30Insulin Signaling PathwayThe insulin transduction pathway is an important biochemical pathway beginning at the cellular level affecting homeostasis. This pathway is also influenced by fed versus fasting states, stress levels, and a variety of other hormones.<br />
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When carbohydrates are consumed, digested, and absorbed the pancreas senses the subsequent rise in blood glucose concentration and releases insulin to promote an uptake of glucose from the blood stream. When insulin binds on the cellular insulin receptor, it leads to a cascade of cellular processes that promote the usage or, in some cases, the storage of glucose in the cell. The effects of insulin vary depending on the tissue involved, e.g., insulin is most important in the uptake of glucose by muscle and adipose tissue.<br />
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This insulin signal transduction pathway is composed of trigger mechanisms (e.g., autophosphorylation mechanisms) that serve as signals throughout the cell. There is also a counter mechanism in the body to stop the secretion of insulin beyond a certain limit. Namely, those counter-regulatory mechanisms are glucagon and epinephrine. The process of the regulation of blood glucose (also known as glucose homeostasis) also exhibits oscillatory behavior.<br />
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On a pathological basis, this topic is crucial to understanding certain disorders in the body such as diabetes (type 1,2,3), hyperglycemia and hypoglycemia.<br />
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<br />
Insulin signal transduction pathway<br />
The functioning of a signal transduction pathway is based on extra-cellular signaling that in turn creates a response which causes other subsequent responses, hence creating a chain reaction, or cascade. During the course of signaling, the cell uses each response for accomplishing some kind of a purpose along the way. Insulin secretion mechanism is a common example of signal transduction pathway mechanism.<br />
<br />
Insulin is produced by the pancreas in a region called Islets of Langerhans. In the islets of Langerhans, there are beta-cells, which are responsible for production of insulin. Insulin is secreted as a response mechanism for counteracting the increasing excess amounts of glucose in the blood.<br />
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Glucose in the body increases after food consumption. This is primarily due to carbohydrate intake, but to much lesser degree protein intake ([1])([2]). Depending on the tissue type, the glucose enters the cell through facilitated or passive diffusion. In muscle and adipose tissue, glucose enters through GLUT 4 receptors via facilitated diffusion ([3]). In brain, kidney and retina, glucose enters passively. In the beta-cells of the pancreas, glucose enters through the GLUT 2 receptors (process described below).<br />
<br />
Two aspects of this process are explained below: insulin secretion and insulin action on the cell.<br />
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Insulin secretion<br />
<br />
The glucose that goes in the bloodstream after food consumption also enters the beta cells in the Islets of Langerhans in the pancreas. The glucose passively diffuses in the beta cell through a GLUT-2 vesicle. Inside the beta cell, the following process occurs:<br />
<br />
Glucose gets converted to Glucose-6-Phosphate (G6P) through Glucokinase; and G6P is subsequently oxidized to form ATP. This process inhibits the ATP sensitive potassium ion channels of the cell causing the Potassium ion channel to close and not function anymore. The closure of the ATP-sensitive potassium channels causes depolarization of the cell membrane causing the cell membrane to stretch which causes the voltage-gated calcium channel on the membrane to open causing an influx of Ca2+ ions. This influx then stimulates fusion of the insulin vesicles (bubble like structure with insulin in them) to the cell membrane and secretion of insulin in the extracellular fluid outside the beta cell; thus making it enter the bloodstream.<br />
<br />
Insulin action on the cell<br />
After insulin enters the bloodstream, it is taken up by the cells, as glucose is the preferential fuel for human bodies. However, insulin does not directly go inside the cell in its original form. To activate the effects of insulin, it has to bind to an enzyme that activates its functions. Thus, the insulin binds to the α (alpha) subunit of the insulin receptor embedded in the cell membrane. The α-subunit acts as the insulin receptor and the insulin molecule acts as a ligand in an receptor-ligand complex.<br />
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This triggers the tyrosine kinase activity in the β-subunit that is attached to the α-subunit. The tyrosine kinase activity causes phosphorylation (activation) of the enzymes.<br />
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The 2 enzymes, Mitogen-activated Protein Kinase (MAP-Kinase) and Phosphatidylinositol-3-Kinase (PI-3K, Phosphoinositide 3-kinase) are responsible for expressing the mitogenic and metabolic actions of Insulin respectively.<br />
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The activation of MAP-Kinase leads to completion of mitogenic functions like cell growth and gene expression.<br />
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The activation of PI-3K leads to crucial metabolic functions such as synthesis of lipids, proteins and glycogen. It also leads to cell survival and cell proliferation. Most importantly, the PI-3K pathway is responsible for the distribution of glucose for important cell functions. The GLUT-4 vesicle (responsible for passive diffusion of glucose in cell) binds to the PI-3K after bringing glucose in the cell. The PI-3K isolates the GLUT-4 Vesicle from the glucose and sends the vesicle back to the cell membrane. The glucose that is isolated is then sent to the mitochondria to make ATP and excess glucose is stored in the cell as glycogen. [This process is also illustrated in Figure 1.1.2].[2]<br />
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Thus, insulin’s role is more of a promoter for the usage of glucose in the cells rather than neutralizing or counteracting it.<br />
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<br />
Wikipedia contributors. "Insulin signal transduction pathway and regulation of blood glucose." Wikipedia, The Free Encyclopedia. Wikipedia, The Free Encyclopedia, 15 Sep. 2015. Web. 25 Oct. 2015.<br />
Julianhttp://www.blogger.com/profile/14658964544887212629noreply@blogger.com2tag:blogger.com,1999:blog-132690756808990032.post-31045909936589195912015-07-02T21:22:00.000+05:302015-07-02T21:22:24.380+05:30Induced Pluripotent stem (iPS ) Cells<div style="text-align: justify;">Induced pluripotent stem cells, commonly abbreviated as iPS cells or iPSCs are a type of pluripotent stem cell artificially derived from a non-pluripotent cell, typically an adult somatic cell, by inducing a "forced" expression of specific genes.</div><br />
<div style="text-align: justify;">Induced Pluripotent Stem Cells are similar to natural pluripotent stem cells, such as embryonic stem (ES) cells, in many respects, such as the expression of certain stem cell genes and proteins, chromatin methylation patterns, doubling time, embryoid body formation, teratoma formation, viable chimera formation, and potency and differentiability, but the full extent of their relation to natural pluripotent stem cells is still being assessed.</div><img src="http://i4.ytimg.com/vi/fGNchPdlaGU/default.jpg"width="10"height="5" alt=" " ><br />
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<div style="text-align: justify;">iPSCs were first produced in 2006 from mouse cells and in 2007 from human cells. This has been cited as an important advance in stem cell research, as it may allow researchers to obtain pluripotent stem cells, which are important in research and potentially have therapeutic uses, without the controversial use of embryos. They may also be less prone to immune rejection than embryonic stem cells because of the fact that they are derived entirely from the patient.</div><br />
<div style="text-align: justify;">Depending on the methods used, reprogramming of adult cells to obtain iPSCs may pose significant risks that could limit its use in humans. For example, if viruses are used to genomically alter the cells, the expression of cancer-causing genes or oncogenes may potentially be triggered. In February 2008, in ground-breaking findings published in the journal Cell, scientists announced the discovery of a technique that could remove oncogenes after the induction of pluripotency, thereby increasing the potential use of iPS cells in human diseases[3]. In April 2009, Sheng Ding and colleagues in La Jolla, California, demonstrated that generation of iPS cells is possible without any genetic alteration of the adult cell: A repeated treatment of the cells with certain proteins channeled into the cells via poly-arginine anchors was sufficient to induce pluripotency. The acronym given for those iPSCs is piPSCs</div>Julianhttp://www.blogger.com/profile/14658964544887212629noreply@blogger.com1tag:blogger.com,1999:blog-132690756808990032.post-18541179741114674292014-11-02T04:52:00.000+05:302014-11-02T04:52:52.668+05:30TamiFlu<div style="text-align: justify;">Tamiflu (oseltamivir phosphate) is an antiviral drug marketed by the Swiss pharmaceutical company Roche. It belongs to a group of drugs called neuraminidase inhibitors and can shorten the duration and lessen the severity of the type A and B strains of the flu, as well as bird flu.</div>How neuraminidase inhibitors works<br />
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<div style="text-align: justify;">Tamiflu targets a protein called neuraminidase that lives on the flu virus cells. This protein helps the flu virus break through the cell walls so it can move on to other cells and replicate itself. Tamiflu inhibits the neuraminidase protein, so that the virus can't leave the cell to infect other cells. Eventually, the virus dies.</div>How Tamiflu kills the virus<br />
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<div style="text-align: justify;">Tamiflu can't stop the flu entirely. However, studies have shown that if you take it within 48 hours of showing symptoms, it can shorten the duration of the flu (strains A and B). Patients with the flu who took it felt better 30 percent (or 1.3 days) faster than people who didn't take it . The drug also can help protect you from getting the flu if you're exposed to someone who has it. But Tamiflu can't prevent the spread of the disease, and it won't stop illnesses (like the common cold) that resemble the flu.</div>Julianhttp://www.blogger.com/profile/14658964544887212629noreply@blogger.com1tag:blogger.com,1999:blog-132690756808990032.post-89787415345539685942014-06-03T17:38:00.000+05:302014-06-06T11:29:36.976+05:30Sorafenib<div style="text-align: justify;">Sorafenib (co-developed and co-marketed by Bayer and Onyx Pharmaceuticals as Nexavar),[1] is a drug approved for the treatment of primary kidney cancer (advanced renal cell carcinoma) and advanced primary liver cancer (hepatocellular carcinoma).</div><br />
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<div style="text-align: justify;">Sorafenib (a bi-aryl urea) is a small molecular inhibitor of several Tyrosine protein kinases (VEGFR and PDGFR) and Raf kinases (more avidly C-Raf than B-Raf).</div><div style="text-align: justify;">(Protein kinases are overactive in many of the molecular pathways that cause cells to become cancerous. These pathways include Raf kinase, PDGF (platelet-derived growth factor), VEGF receptor 2 and 3 kinases and c Kit the receptor for Stem cell factor. )</div><div style="text-align: justify;">Sorafenib is/was unique in targeting the Raf/Mek/Erk pathway (MAP Kinase pathway).</div>Sorafenib inhibits some intracellular serine/threonine kinases (e.g. C-Raf, wild-type B-Raf and mutant B-Raf).Julianhttp://www.blogger.com/profile/14658964544887212629noreply@blogger.com0tag:blogger.com,1999:blog-132690756808990032.post-6870958328250608752014-04-03T12:56:00.000+05:302014-04-03T12:57:00.609+05:30Protein G B1 domain<img src="http://i4.ytimg.com/vi/2NCIgk2cH44/default.jpg" height="5" width="10" /> <iframe allowfullscreen="" frameborder="0" height="480" src="https://www.youtube.com/embed/2NCIgk2cH44?rel=0" width="640"></iframe> <br />
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</script></div><div style="text-align: justify;">The solution structure of the isolated fragments 1-20 (beta-hairpin), 21-40 (alpha-helix) and 41-56 (beta-hairpin), corresponding to all the secondary structure elements of the protein G B1 domain, have been studied by circular dichroism and nuclear magnetic resonance techniques. In the protein G B1-(1-20) fragment turn-like folded structures were detected in water though low populated. In the presence of 30% aqueous trifluoroethanol there is a complex conformational behaviour in which a helical structure at the N-terminal half is formed in equilibrium with random and native-like beta-hairpin structures. The peptide corresponding to the alpha-helix is predominantly unstructured in water, while in 30% trifluoroethanol it highly populates a native alpha-helical conformation, including a (i,i + 5) interaction between hydrophobic residues at its C-terminus. The third peptide was previously reported to form a monomeric native beta-hairpin structure in water . We show in this work that the beta-hairpin structure is further stabilized in 30% trifluoroethanol and destabilised in the presence of 6 M urea, though some folded structure persists even in these highly denaturing conditions. The conformational properties of these peptides suggests that the second beta-hairpin could be an important folding initiation site on which the rest of the chain folds. Reconstitution experiments failed to show evidence of interaction between the peptides. Algorithms designed to predict the helical and extended conformations of peptides in aqueous solution successfully describe the complicated behaviour of these peptides. Comparison of the predicted and the experimental results with those for a structurally related protein, ubiquitin, shows very strong similarities, the main difference being the switch of the most stable beta-hairpin from the N-terminus in ubiquitin to the C-terminus in protein G.</div>Julianhttp://www.blogger.com/profile/14658964544887212629noreply@blogger.comtag:blogger.com,1999:blog-132690756808990032.post-41858975910953879532014-01-22T11:43:00.000+05:302014-01-22T11:44:29.416+05:30Mast cell Animation<div style="text-align: justify;">A mast cell (or mastocyte) is a resident cell of several types of tissues and contains many granules rich in histamine and heparin. Although best known for their role in allergy and anaphylaxis, mast cells play an important protective role as well, being intimately involved in wound healing and defense against pathogens.</div><img src="http://video.google.com/ThumbnailServer2?app=blogger&contentid=615a3b362f24b845&offsetms=5000&itag=w160&sigh=ynCCuS4upKidsq-wiPh0SyK733o"width="10"height="5" alt=" " ><br />
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<div style="text-align: justify;">Mast cells were first described by Paul Ehrlich in his 1878 doctoral thesis on the basis of their unique staining characteristics and large granules. These granules also led him to the mistaken belief that they existed to nourish the surrounding tissue, and he named them "mastzellen," a German term, meaning "feeding-cells." Nowadays, they are considered part of the immune system. Mast cells are very similar to basophil granulocytes (a class of white blood cells) in blood; the similarities between mast cells and basophils has led many to speculate that mast cells are basophils that have "homed in" on tissues. However, current evidence suggests that they are generated by different precursor cells in the bone marrow. Nevertheless, both mast cells and basophils are thought to originate from bone marrow precursors expressing the CD34 molecule. The basophil leaves the bone marrow already mature while the mast cell circulates in an immature form, only maturing once in a tissue site. The tissue site an immature mast cell chooses to settle in probably determines its precise characteristics.</div><br />
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<br />
<div style="text-align: justify;">Two types of mast cells are recognized, those from connective tissue and a distinct set of mucosal mast cells. The activities of the latter are dependent on T-cells.</div><br />
<div style="text-align: justify;">Mast cells are present in most tissues in the vicinity of blood vessels, and are especially prominent near the boundaries between the outside world and the internal milieu, such as the skin, mucosa of the lungs and digestive tract, as well as in the mouth, conjunctiva and nose.</div><br />
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<span style="font-weight: bold;">Physiology</span><br />
<div style="text-align: justify;">Mast cells play a key role in the inflammatory process. When activated, a mast cell rapidly releases its characteristic granules and various hormonal mediators into the interstitium. Mast cells can be stimulated to degranulate by direct injury (e.g physical or chemical), cross-linking of IgE receptors, or by activated complement proteins.</div><br />
<div style="text-align: justify;">Mast cells express a high-affinity receptor (FcεRI) for the Fc region of Immunoglobulin E (IgE), the least-abundant member of the antibodies. This receptor is of such high affinity that binding of IgE molecules is essentially irreversible. As a result, mast cells are coated with IgE. IgE is produced by B-cells (the antibody-producing cells of the immune system). IgE molecules, like all antibodies, are specific to one particular antigen.</div><br />
<div style="text-align: justify;">In allergic reactions, mast cells remain inactive until an allergen binds to IgE already in association with the cell (see above). Allergens are generally proteins or polysaccharides. The allergen binds to the Fab part of the IgE molecules on the mast cell surface. It appears that binding of two or more IgE molecules (this is called crosslinking) is required to activate the mast cell; the steric changes lead to a slight disturbance to the cell membrane structure, causing a complex sequence of reactions inside the cell that lead to its activation. Although this reaction is most well understood in terms of allergy, it appears to have evolved as a defense system against intestinal worm infestations (tapeworms, etc).</div><br />
The molecules thus released into the intercellular environment include:<br />
<br />
<ul><li> preformed mediators (from the granules):</li>
<li> histamine (2-5 pg/cell)</li>
<li> proteoglycans, mainly heparin (active as anticoagulant)</li>
<li> serine proteases</li>
<li> newly formed lipid mediators (eicosanoids):</li>
<li> prostaglandin D2</li>
<li> leukotriene C4</li>
<li> cytokines</li>
</ul><br />
<div style="text-align: justify;">Histamine dilates post capillary venules, activates the endothelium, and increases blood vessel permeability. This leads to local edema (swelling), warmth, redness, and the attraction of other inflammatory cells to the site of release. It also irritates nerve endings (leading to itching or pain). Cutaneous signs of histamine release are the "flare and wheal"-reaction. The bump and redness immediately following a mosquito bite are a good example of this reaction, which occurs seconds after challenge of the mast cell by an allergen.</div><br />
<div style="text-align: justify;">The other physiologic activities of mast cells are much less well-understood. Several lines of evidence suggest that mast cells may have a fairly fundamental role in innate immunity -- they are capable of elaborating a vast array of important cytokines and other inflammatory mediators, they express multiple "pattern recognition receptors" thought to be involved in recognizing broad classes of pathogens, and mice without mast cells seem to be much more susceptible to a variety of infections.</div>Julianhttp://www.blogger.com/profile/14658964544887212629noreply@blogger.com0tag:blogger.com,1999:blog-132690756808990032.post-85634512874447362702013-11-16T22:11:00.003+05:302016-09-09T23:02:27.692+05:3050-year quest to replace warfarin: Nature Video<div dir="ltr" style="text-align: left;" trbidi="on"><img alt=" " src="http://i4.ytimg.com/vi/6d2vODRA33A/default.jpg" height="5" width="10" /><br />
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<div style="text-align: justify;">In this form of transport, molecules move against either an electrical or concentration gradient (collectively termed an electrochemical gradient).</div><ul style="text-align: justify;"><li>The active transport of small molecules or ions across a cell membrane is generally carried out by transport proteins that are found in the membrane. </li>
</ul><ul style="text-align: justify;"><li>Larger molecules such as starch can also be actively transported across the cell membrane by processes known as endocytosis and exocytosis. </li>
</ul><ul><li><div style="text-align: justify;">The process whereby particles are moved through a membrane from a region of low concentration to a region of high concentration is known as active transport</div><b></b></li>
</ul><br />
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<b>Relation to Cellular Energy</b> <br />
<div align="justify">Using conservative assumptions, it has been calculated that the amount of energy available to the cell is insufficient to power the estimated degree of active transport, e.g. by a factor of 15-30 times for the sodium pump alone . Following vigorous debate in Science and elsewhere, this discrepancy has yet to be resolved. </div>Julianhttp://www.blogger.com/profile/14658964544887212629noreply@blogger.com0tag:blogger.com,1999:blog-132690756808990032.post-24183885244974659892013-11-05T18:18:00.000+05:302013-11-05T18:21:08.526+05:30Plasmid DNA Extraction<div style="text-align: justify;">Plasmid is a DNA molecule that is separate from, and can replicate independently of, the chromosomal DNA.[1] They are double stranded and, in many cases, circular. Plasmids usually occur naturally in bacteria, but are sometimes found in eukaryotic organisms .<br />
<br />
<div style="text-align: justify;">Plasmids are often used to purify a specific sequence, since they can easily be purified away from the rest of the genome. For their use as vectors, and for molecular cloning, plasmids often need to be isolated.</div></div><br />
<div style="text-align: justify;">There are several methods to isolate plasmid DNA from bacteria, the archetypes of which are the miniprep and the maxiprep/bulkprep.The former can be used to quickly find out whether the plasmid is correct in any of several bacterial clones. The yield is a small amount of impure plasmid DNA, which is sufficient for analysis by restriction digest and for some cloning techniques.</div><br />
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<div style="text-align: justify;">In the latter, much larger volumes of bacterial suspension are grown from which a maxi-prep can be performed. Essentially this is a scaled-up miniprep followed by additional purification. This results in relatively large amounts (several micrograms) of very pure plasmid DNA.</div><img src="http://video.google.com/ThumbnailServer2?app=blogger&contentid=a19bcf4a8545383b&offsetms=5000&itag=w160&sigh=h65APymDxga64P3S4zgprmYTlbs"width="10"height="5" alt=" " >Julianhttp://www.blogger.com/profile/14658964544887212629noreply@blogger.com0tag:blogger.com,1999:blog-132690756808990032.post-32459613131524445192013-11-04T21:17:00.001+05:302013-11-04T21:17:22.050+05:30Multiple Sclerosis Animation<div style="text-align: justify;">Multiple sclerosis (abbreviated MS, also known as disseminated sclerosis or encephalomyelitis disseminata) is an autoimmune condition in which the immune system attacks the central nervous system, leading to demyelination. Disease onset usually occurs in young adults, and it is more common in women. It has a prevalence that ranges between 2 and 150 per 100,000. MS was first described in 1868 by Jean-Martin Charcot.</div><div style="text-align: justify;">MS affects the ability of nerve cells in the brain and spinal cord to communicate with each other. Nerve cells communicate by sending electrical signals called action potentials down long fibers called axons, which are wrapped in an insulating substance called myelin. In MS, the body's own immune system attacks and damages the myelin. When myelin is lost, the axons can no longer effectively conduct signals. The name multiple sclerosis refers to scars (scleroses – better known as plaques or lesions) in the white matter of the brain and spinal cord, which is mainly composed of myelin.Although much is known about the mechanisms involved in the disease process, the cause remains unknown. Theories include genetics or infections. Different environmental risk factors have also been found.</div><iframe width="640" height="480" src="https://www.youtube.com/embed/qgySDmRRzxY?rel=0" frameborder="0" allowfullscreen></iframe> <br />
<script type="text/javascript">var addthis_pub="j_thomas";</script><a expr:name='data:post.title' expr:id='data:post.url' onmouseover='return addthis_open(this, "", this.id, this.name);' onmouseout='addthis_close()' onclick='return addthis_sendto()'><img src="http://s7.addthis.com/static/btn/lg-addthis-en.gif" width="125" height="16" border="0" alt="" style="border:0"/></a><script type="text/javascript" src="http://s7.addthis.com/js/152/addthis_widget.js"></script> <a href="http://feedproxy.google.com/Biosolutions" rel="alternate" type="application/rss+xml"><img alt="" style="vertical-align:middle;border:0" src="http://www.feedburner.com/fb/images/pub/feed-icon32x32.png"/></a> <a href="http://feedproxy.google.com/Biosolutions" rel="alternate" type="application/rss+xml">Subscribe in a reader</a> <br />
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</script></div><div style="text-align: justify;">Multiple sclerosis (MS) is a disease that affects the central nervous system. the CNS which consist of the brain spinal cord optic nerves everything we do whether it's taking a step to solving a problem or simply breathing relies on the proper functioning of the CNS.To understand how MS may impact the CNS we must explore the disease at the cellular level .In the brain millions of nerve cells called neurons continually send and receive signals, each signal is a minute but necessary part of the intricate CNS orchestrations that culminate in the actions, sensations, thoughts and emotions that comprised the human experience.Normally the path over which a nerve signal travels is protected by a type of insulation called the myelin sheath,this insulation is essential for nerve signals to reach their target.In MS the myelin sheath is eroded and the underlying wire like nerve fiber is also damaged, this leads to a breakdown in the ability of the nerve cells transmit signals. it is believed that the loss of myelin is the result of mistaken attack of immune cells.Immune cells protect the body against foreign substances such as bacteria and viruses .But in MS something goes awry.Immune cells infiltrate the brain and spinal cord seek and attack.As ongoing inflammation and tissue damage occurs nerve signals are disrupted. this causes unpredictable symptoms that can range from numbness or tingling to blindness and paralysis.These losses may be temporary or permanent</div>Julianhttp://www.blogger.com/profile/14658964544887212629noreply@blogger.comtag:blogger.com,1999:blog-132690756808990032.post-79203960530819545172013-11-01T02:07:00.001+05:302016-09-10T00:01:06.384+05:30DNA Extraction<div style="text-align: justify;">DNA extraction is a routine procedure to collect DNA for subsequent molecular or forensic analysis. There are three basic steps in a DNA extraction:<br />
</div><br />
<ol><li style="text-align: justify;">Breaking the cells open to expose the DNA within, such as by grinding or sonicating the sample.</li>
<li style="text-align: justify;">Removing membrane lipids by adding a detergent.</li>
<li style="text-align: justify;">Precipitating the DNA with an alcohol — usually ethanol or isopropanol. Since DNA is insoluble in these alcohols, it will aggregate together, giving a pellet on centrifugation. This step also removes alcohol-soluble salt.</li>
</ol><img src="http://i2.ytimg.com/vi/Q53NRh_KJ6w/2.jpg"width="10" height="5" ><br />
<br />
<div style="text-align: justify;">Refinements of the technique include adding a chelating agent to sequester divalent cations such as Mg2+ and Ca2+. This stops dnase enzymes from degrading the DNA.<br />
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<div style="text-align: justify;">Cellular and histone proteins bound to the DNA can be removed prior to its precipitation either by adding a protease or having prior to precipitation, precipitating with sodium or ammonium acetate, or extracting with a phenol-chloroform mixture.<br />
</div><br />
<div style="text-align: justify;">If desired, the DNA can be resolubilized in a slightly alkaline buffer.<br />
</div><br />
<br />
<span style="font-weight: bold;">Detecting DNA</span><br />
<br />
<div style="text-align: justify;">A diphenylamine (DPA) indicators will confirm the presence of DNA. This procedure involves chemical hydrolysis of DNA: when heated (e.g. ≥95oC) in acid, the reaction requires a deoxyribose sugar and therefore is specific for DNA. Under these conditions, the 2-deoxyribose is converted to w-hydroxylevulinyl aldehyde, which reacts with the compound, diphenylamine, to produce a blue-colored compound. DNA concentration can be determined measuring the intensity of absorbance of the solution at the 600 nm with a spectrophotometer and comparing to a standard curve of known DNA concentrations.<br />
</div><br />
<div style="text-align: justify;">Measuring the intensity of absorbance of the DNA solution at wavelengths 260 nm and 280nm is used as a measure of DNA purity. DNA absorbs UV light at 260 and 280 nm, and aromatic proteins absorbs UV light at 280 nm; a pure sample of DNA has the 260/280 ratio at 1.8 and is relatively free from protein contamination. A DNA preparation that is contaminated with protein will have a 260/280 ratio lower than 1.8.<br />
</div><br />
<div style="text-align: justify;">DNA can be quantified by cutting the DNA with a restriction enzyme, running it on an agarose gel, staining with ethidium bromide or a different stain and comparing the intensity of the DNA with a DNA marker of known concentration.<br />
</div><br />
<div style="text-align: justify;">Using the Southern blot technique this quantified DNA can be isolated and examined further using PCR and RFLP analysis. These procedures allow differentiation of the repeated sequences within the genome. It is these techniques which forensic scientists use for comparison and identification.</div>Julianhttp://www.blogger.com/profile/14658964544887212629noreply@blogger.com0tag:blogger.com,1999:blog-132690756808990032.post-50779714886334691502013-11-01T02:07:00.000+05:302016-09-10T00:09:35.081+05:30Western Blot Animation<div dir="ltr" style="text-align: left;" trbidi="on"><div align="justify">The western blot (alternately, immunoblot) is a method of detecting specific proteins in a given sample of tissue homogenate or extract. It uses gel electrophoresis to separate native or denatured proteins by the length of the polypeptide (denaturing conditions) or by the 3-D structure of the protein (native/ non-denaturing conditions). The proteins are then transferred to a membrane (typically nitrocellulose or PVDF), where they are probed (detected) using antibodies specific to the target protein. There are now many reagent companies that specialize in providing antibodies (both monoclonal and polyclonal antibodies) against many thousands of different proteins. Commercial antibodies can be expensive, though the unbound antibody can be reused between experiments. This method is used in the fields of molecular biology, biochemistry, immunogenetics and other mo<br />
<br />
Molecular biology disciplines. <br />
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Other related techniques include using antibodies to detect proteins in tissues and cells by immunostaining and enzyme-linked immunosorbent assay (ELISA). The method originated from the laboratory of George Stark at Stanford. The name western blot was given to the technique by W. Neal Burnette and is a play on the name Southern blot, a technique for DNA detection developed earlier by Edwin Southern. Detection of RNA is termed northern blotting. <br />
<span style="font-weight: bold;"> Steps in a Western blot Tissue preparation</span><br />
<br />
Samples may be taken from whole tissue or from cell culture. In most cases, solid tissues are first broken down mechanically using a blender (for larger sample volumes), using a homogenizer (smaller volumes), or by sonication. Cells may also be broken open by one of the above mechanical methods. However, it should be noted that bacteria, virus or environmental samples can be the source of protein and thus Western blotting is not restricted to cellular studies only. Assorted detergents, salts, and buffers may be employed to encourage lysis of cells and to solubilize proteins. Protease and phosphatase inhibitors are often added to prevent the digestion of the sample by its own enzymes. A combination of biochemical and mechanical techniques – including various types of filtration and centrifugation – can be used to separate different cell compartments and organelles.<br />
<br />
<b> Gel electrophoresis</b><br />
The proteins of the sample are separated using gel electrophoresis. Separation of proteins may be by isoelectric point (pI), molecular weight, electric charge, or a combination of these factors. The nature of the separation depends on the treatment of the sample and the nature of the gel. By far the most common type of gel electrophoresis employs polyacrylamide gels and buffers loaded with sodium dodecyl sulfate (SDS). SDS-PAGE (SDS polyacrylamide gel electrophoresis) maintains polypeptides in a denatured state once they have been treated with strong reducing agents to remove secondary and tertiary structure (e.g. S-S disulfide bonds to SH and SH) and thus allows separation of proteins by their molecular weight. Sampled proteins become covered in the negatively charged SDS and move to the positively charged electrode through the acrylamide mesh of the gel. Smaller proteins migrate faster through this mesh and the proteins are thus separated according to size (usually measured in kilo Daltons, kD). The concentration of acrylamide determines the resolution of the gel - the greater the acrylamide concentration the better the resolution of lower molecular weight proteins. The lower the acrylamide concentration the better the resolution of higher molecular weight proteins. Proteins travel only in one dimension along the gel for most blots. Samples are loaded into wells in the gel. One lane is usually reserved for a marker or ladder, a commercially available mixture of proteins having defined molecular weights, typically stained so as to form visible, coloured bands. An example of a ladder is the GE Full Range Molecular weight ladder (Figure 1). When voltage is applied along the gel, proteins migrate into it at different speeds. These different rates of advancement (different electrophoretic mobilities) separate into bands within each lane. It is also possible to use a two-dimensional (2-D) gel which spreads the proteins from a single sample out in two dimensions. Proteins are separated according to isoelectric point (pH at which they have neutral net charge) in the first dimension, and according to their molecular weight in the second dimension.<br />
<br />
<b>Transfer</b> <br />
<br />
In order to make the proteins accessible to antibody detection, they are moved from within the gel onto a membrane made of nitrocellulose or polyvinylidene fluoride (PVDF). The membrane is placed on top of the gel, and a stack of tissue papers placed on top of that. The entire stack is placed in a buffer solution which moves up the paper by capillary action, bringing the proteins with it. Another method for transferring the proteins is called electroblotting and uses an electric current to pull proteins from the gel into the PVDF or nitrocellulose membrane. The proteins move from within the gel onto the membrane while maintaining the organization they had within the gel. As a result of this "blotting" process, the proteins are exposed on a thin surface layer for detection (see below). Both varieties of membrane are chosen for their non-specific protein binding properties (i.e. binds all proteins equally well). Protein binding is based upon hydrophobic interactions, as well as charged interactions between the membrane and protein. Nitrocellulose membranes are cheaper than PVDF, but are far more fragile and do not stand up well to repeated probings. The uniformity and overall effectiveness of transfer of protein from the gel to the membrane can be checked by staining the membrane with Coomassie or Ponceau S dyes. Coomassie is the more sensitive of the two, although Ponceau S's water solubility makes it easier to subsequently destain and probe the membrane as described below. <br />
<b>Blocking</b> <br />
Since the membrane has been chosen for its ability to bind protein, and both antibodies and the target are proteins, steps must be taken to prevent interactions between the membrane and the antibody used for detection of the target protein. Blocking of non-specific binding is achieved by placing the membrane in a dilute solution of protein - typically Bovine serum albumin (BSA) or non-fat dry milk (both are inexpensive), with a minute percentage of detergent such as Tween 20. The protein in the dilute solution attaches to the membrane in all places where the target proteins have not attached. Thus, when the antibody is added, there is no room on the membrane for it to attach other than on the binding sites of the specific target protein. This reduces "noise" in the final product of the Western blot, leading to clearer results, and eliminates false positives. <br />
<br />
<b>Detection</b><br />
During the detection process the membrane is "probed" for the protein of interest with a modified antibody which is linked to a reporter enzyme, which when exposed to an appropriate substrate drives a colourimetric reaction and produces a colour. For a variety of reasons, this traditionally takes place in a two-step process, although there are now one-step detection methods available for certain applications. <br />
<b>Two step</b> <br />
<b>Primary antibody</b> <br />
Antibodies are generated when a host species or immune cell culture is exposed to the protein of interest (or a part thereof). Normally, this is part of the immune response, whereas here they are harvested and used as sensitive and specific detection tools that bind the protein directly. After blocking, a dilute solution of primary antibody (generally between 0.5 and 5 micrograms/ml) is incubated with the membrane under gentle agitation. Typically, the solution is composed of buffered saline solution with a small percentage of detergent, and sometimes with powdered milk or BSA. The antibody solution and the membrane can be sealed and incubated together for anywhere from 30 minutes to overnight. It can also be incubated at different temperatures, with warmer temperatures being associated with more binding, both specific (to the target protein, the "signal") and non-specific ("noise"). <br />
<b>Secondary Antibody</b> <br />
After rinsing the membrane to remove unbound primary antibody, the membrane is exposed to another antibody, directed at a species-specific portion of the primary antibody. This is known as a secondary antibody, and due to its targeting properties, tends to be referred to as "anti-mouse," "anti-goat," etc. Antibodies come from animal sources (or animal sourced hybridoma cultures); an anti-mouse secondary will bind to just about any mouse-sourced primary antibody. This allows some cost savings by allowing an entire lab to share a single source of mass-produced antibody, and provides far more consistent results. The secondary antibody is usually linked to biotin or to a reporter enzyme such as alkaline phosphatase or horseradish peroxidase. This means that several secondary antibodies will bind to one primary antibody and enhances the signal. Most commonly, a horseradish peroxidase-linked secondary is used in conjunction with a chemiluminescent agent, and the reaction product produces luminescence in proportion to the amount of protein. A sensitive sheet of photographic film is placed against the membrane, and exposure to the light from the reaction creates an image of the antibodies bound to the blot. As with the ELISPOT and ELISA procedures, the enzyme can be provided with a substrate molecule that will be converted by the enzyme to a colored reaction product that will be visible on the membrane . A third alternative is to use a radioactive label rather than an enzyme coupled to the secondary antibody, such as labeling an antibody-binding protein like Staphylococcus Protein A with a radioactive isotope of iodine. Since other methods are safer, quicker and cheaper this method is now rarely used.<br />
<br />
<b>One step</b> <br />
Historically, the probing process was performed in two steps because of the relative ease of producing primary and secondary antibodies in separate processes. This gives researchers and corporations huge advantages in terms of flexibility, and adds an amplification step to the detection process. Given the advent of high-throughput protein analysis and lower limits of detection, however, there has been interest in developing one-step probing systems that would allow the process to occur faster and with less consumables. This requires a probe antibody which both recognizes the protein of interest and contains a detectable label, probes which are often available for known protein tags. The primary probe is incubated with the membrane in a manner similar to that for the primary antibody in a two-step process, and then is ready for direct detection after a series of wash steps. <br />
<b>Analysis</b> <br />
After the unbound probes are washed away, the Western blot is ready for detection of the probes that are labeled and bound to the protein of interest. In practical terms, not all Westerns reveal protein only at one band in a membrane. Size approximations are taken by comparing the stained bands to that of the marker or ladder loaded during electrophoresis. The process is repeated for a structural protein, such as actin or tubulin, that should not change between samples. The amount of target protein is indexed to the structural protein to control between groups. This practice ensures correction for the amount of total protein on the membrane in case of errors or incomplete transfers. <br />
<b>Colorimetric detection</b> <br />
The colorimetric detection method depends on incubation of the Western blot with a substrate that reacts with the reporter enzyme (such as peroxidase) that is bound to the secondary antibody. This converts the soluble dye into an insoluble form of a different color that precipitates next to the enzyme and thereby stains the membrane. Development of the blot is then stopped by washing away the soluble dye. Protein levels are evaluated through densitometry (how intense the stain is) or spectrophotometry. <br />
<br />
<b>Chemiluminescence</b> <br />
Chemiluminescent detection methods depend on incubation of the Western blot with a substrate that will luminesce when exposed to the reporter on the secondary antibody. The light is then detected by photographic film, and more recently by CCD cameras which captures a digital image of the Western blot. The image is analysed by densitometry, which evaluates the relative amount of protein staining and quantifies the results in terms of optical density. Newer software allows further data analysis such as molecular weight analysis if appropriate standards are used. So-called "enhanced chemiluminescent" (ECL) detection is considered to be among the most sensitive detection methods for blotting analysis. <br />
<br />
<b>Radioactive detection</b> <br />
<br />
Radioactive labels do not require enzyme substrates, but rather allow the placement of medical X-ray film directly against the Western blot which develops as it is exposed to the label and creates dark regions which correspond to the protein bands of interest . The importance of radioactive detections methods is declining, because it is very expensive, health and safety risks are high and ECL provides a useful alternative. <br />
<br />
<b>Fluorescent detection</b> <br />
<br />
The fluorescently labeled probe is excited by light and the emission of the excitation is then detected by a photosensor such as CCD camera equipped with appropriate emission filters which captures a digital image of the Western blot and allows further data analysis such as molecular weight analysis and a quantitative Western blot analysis. Fluorescence is considered to be among the most sensitive detection methods for blotting analysis. <br />
<br />
<b>Secondary probing</b> <br />
One major difference between nitrocellulose and PVDF membranes relates to the ability of each to support "stripping" antibodies off and reusing the membrane for subsequent antibody probes. While there are well-established protocols available for stripping nitrocellulose membranes, the sturdier PVDF allows for easier stripping, and for more reuse before background noise limits experiments. Another difference is that, unlike nitrocellulose, PVDF must be soaked in 95% ethanol, isopropanol or methanol before use. PVDF membranes also tend to be thicker and more resistant to damage during use. <br />
<br />
<b>2-D Gel Electrophoresis</b> <br />
<br />
2-dimensional SDS-PAGE uses the principles and techniques outlined above. 2-D SDS-PAGE, as the name suggests, involves the migration of polypeptides in 2 dimensions. For example, in the first dimension polypeptides are separated according to isoelectric point, while in the second dimension polypeptides are separated according to their molecular weight. The isoelectric point of a given protein is determined by the relative number of positively (e.g. lysine and arginine) and negatively (e.g. glutamate and aspartate) charged amino acids, with negatively charged amino acids contributing to a high isoelectric point and positively charged amino acids contributing to a low isoelectric point. Samples could also be separated first under nonreducing conditions using SDS-PAGE and under reducing conditions in the second dimension, which breaks apart disulfide bonds that hold subunits together. SDS-PAGE might also be coupled with urea-PAGE for a 2-dimensional gel. In principle, this method allows for the separation of all cellular proteins on a single large gel. A major advantage of this method is that it often distinguishes between different isoforms of a particular protein - e.g. a protein that has been phosphorylated (by addition of a negatively charged group). Proteins that have been separated can be cut out of the gel and then analysed by mass spectrometry, which identifies the protein. <br />
<b>Medical diagnostic applications</b><br />
The confirmatory HIV test employs a Western blot to detect anti-HIV antibody in a human serum sample. Proteins from known HIV-infected cells are separated and blotted on a membrane as above. Then, the serum to be tested is applied in the primary antibody incubation step; free antibody is washed away, and a secondary anti-human antibody linked to an enzyme signal is added. The stained bands then indicate the proteins to which the patient's serum contains antibody. A Western blot is also used as the definitive test for Bovine spongiform encephalopathy (BSE, commonly referred to as 'mad cow disease'). Some forms of Lyme disease testing employ Western blotting.</div></div>Julianhttp://www.blogger.com/profile/14658964544887212629noreply@blogger.com1